Results: Formation of Spheroids From a Primary Colon Cancer Cell Culture, the Effects of Treatments on Spheroids, and the Follow-Up Over Time
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Conclusion
Transcript
Our protocol is significant because it describes a generation of 3D tumor culture models from primary cancer cells, and this model represent real-world tumor biology better than cell lines. This approach is applicable to a variety of solid tumors.
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The present protocol describes generating 3D tumor culture models from primary cancer cells and evaluating their sensitivity to drugs using cell-viability assays and microscopic examinations.