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JoVE Journal

Developmental Biology

This content is Open Access.

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

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08:15 min

November 15th, 2019

November 15th, 2019

5,825 Views

0:04

Title

0:52

Isolation of Fetal Small Intestinal Organoids

3:21

Culturing of Fetal Organoids and Maturation Analysis at RNA and Protein Level

4:42

Enzyme Activity Analysis

5:52

Results: Fetal Organoid Culture

7:22

Conclusion

Transcript

This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days.

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