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Combining Laser Capture Microdissection and Microfluidic qPCR to Analyze Transcriptional Profiles of Single Cells: A Systems Biology Approach to Opioid Dependence

5.2K Views

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09:54 min

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March 8th, 2020

DOI :

10.3791/60612-v

March 8th, 2020

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5,151 Views

Sean J. O'Sullivan¹^,², Beverly A.S. Reyes³, Rajanikanth Vadigepalli¹, Elisabeth J. Van Bockstaele³, James S. Schwaber¹

¹Daniel Baugh Institute for Functional Genomics and Computational Biology, Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, ²Sidney Kimmel Medical College, Thomas Jefferson University, ³Department of Pharmacology & Physiology, Drexel University College of Medicine

Capitoli

Trascrizione

Riepilogo

Esplora Altri Video

Laser Capture Microdissection

Microfluidic QPCR

Transcriptional Profiles

Systems Biology

Opioid Dependence

Gene Expression

Cellular Subphenotypes

Anatomic Specificity

Biological Problems

Ethanol Dehydration

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