Generation of Cancer Cells Stably Expressing Luciferase and a Fluorescent Protein
1:59
Tail Vein Injection of Labeled Cancer Cells
4:17
Monitoring the Metastatic Burden by Bioluminescence with an in vivo Live Animal Imaging Device
6:18
Quantification of the Number and Size of Metastases and Analysis of the Imaging Data
8:01
Results: YAP and TAZ are Required for Metastatic Colonization and Subsequent Growth of Breast Cancer Cells in Syngeneic Mice
9:36
Conclusion
Transcript
Our protocol can be used to quickly and easily test the role of any candidate gene in metastatic colonization and growth. The protocol also allows realtime monitoring of metastasis, formation, and growth, as well as quantification of metastasis nu
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The described approach combines experimental tail vein metastasis assays with in vivo live animal imaging to allow real-time monitoring of breast cancer metastasis formation and growth in addition to the quantification of metastasis number and size in the lungs.