Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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12:11 min
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February 27th, 2020
DOI :
February 27th, 2020
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Chapters in this video
0:04
Introduction
1:19
Desalting of Non-Enriched Proteolytic Peptides via Large-Scale Solid-Phase Extraction
2:46
Simultaneous Enrichment of K-acetylated and K-succinylated Peptides with Immunoaffinity Beads
4:30
Elution of the Peptides Bound to Antibody Beads
7:02
Data Acquisition Using DDA (Data-Dependent Acquisition) and DIA (Data-Independent Acquisition)
10:02
Results: One-pot Enrichment of PTMs
11:19
Conclusion
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