JoVE Logo

Sign In

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

2.2K Views

09:16 min

May 4th, 2022

DOI :

10.3791/63735-v

May 4th, 2022


Explore More Videos

N glycopeptides

Chapters in this video

0:05

Introduction

1:12

Tissue Cryo-Pulverization, Lysis, Protein Extraction

3:35

Protein Digestion and Desalting

5:36

Dual-Functional Ti(IV) Simultaneous Enrichment

7:19

Results: Dual-Functional Ti(IV)-IMAC Enrichment and Mass Spectrometry Analysis of Protein N-Glycosylation and Phosphorylation in Pancreatic Tissues

8:37

Conclusion

Related Videos

article

14:02

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

12.2K Views

article

12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

12.0K Views

article

11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

14.8K Views

article

11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

9.4K Views

article

20:23

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

16.0K Views

article

09:28

Chemical Affinity-Based Isolation of Extracellular Vesicles from Biofluids for Proteomics and Phosphoproteomics Analysis

2.8K Views

article

10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

868 Views

article

10:11

Glycopeptide Capture for Cell Surface Proteomics

11.2K Views

article

09:30

Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

12.8K Views

article

02:02

Phosphopeptide Enrichment: An Antibody-based Immunoprecipitation Technique to Separate Specific Phosphorylated Peptides from Complex Peptide Mixtures

1.1K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved