Generation of a Human iPSC-Based Blood-Brain Barrier Chip
12.3K Views
•
10:20 min
•
March 2nd, 2020
DOI :
March 2nd, 2020
•Srikanth Jagadeesan1,2,3, Michael J. Workman4, Anna Herland5,6, Clive N. Svendsen4, Gad D. Vatine1,2,3
1The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2The Regenerative Medicine and Stem Cell (RMSC) Research Center, Ben-Gurion University of the Negev, 3The Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, 4The Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, 5Division of Micro and Nanosystems, KTH Royal Institute of Technology, 6AIMES, Department of Neuroscience, Karolinska Institutet
Chapters in this video
0:05
Introduction
1:27
Seeding the "Brain Side" Channel and Differentiating EZ Spheres into Mixed Neural Cultures
3:04
Seeding iBMECs (iPSC-Derived Brain Microvascular Endothelial Cells) into the Bottom Channel to Generate the "Blood Side"
5:27
Initiation of Flow
8:12
Results: Immunocytochemistry and Permeability of the iPSC-Base Blood-Brain Barrier Chip
9:17
Conclusion
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