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Developmental Biology

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Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues

5.9K Views

10:14 min

February 27th, 2021

February 27th, 2021

5,900 Views

0:09

Introduction

0:39

Sample Fixation and Dehydration

2:17

LR-White Resin Embedding

3:26

Sample Trimming and Sectioning

5:12

Immunolocalization

7:31

Results: Subcellular Localization of Proteins and Pectins

9:32

Conclusion

Transcript

The amount of information from only one experiment is enormous. We can detect the presence of these cell wall polymers and know exactly how they are distributed in cells and tissues, and even determine their abundance. This method is designed in s

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This protocol describes in detail how the plant material for immunolocalization of Arabinogalactan proteins and pectins is fixed, embedded in a hydrophilic acrylic resin, sectioned and mounted on glass slides. We show cell wall related epitopes will be detected with specific antibodies.

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