Results: Representative Cortical Astrocyte Segmentation and Arborization
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Conclusion
Transcription
In Utero electroporation of MAGIC Marker is a powerful and versatile technique for quickly individualizing and assessing the morphology of cortical astrocyte. The main advantage of this particular technique is to label multiple progenitor cells an
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Astrocytes tile the cerebral cortex uniformly, making the analysis of their complex morphology challenging at the cellular level. The protocol provided here uses multicolor labeling based on in utero electroporation to single out cortical astrocytes and analyze their volume and morphology with a user-friendly image analysis pipeline.