September 12th, 2020
•Here, we introduce and describe a nonradioactive and noninvasive method to assess de novo protein synthesis in vivo, utilizing the nematode Caenorhabditis elegans and fluorescence recovery after photobleaching (FRAP). This method can be combined with genetic and/or pharmacological screens to identify novel modulators of protein synthesis.
Tags
Related Videos
Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism
Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
Basic Caenorhabditis elegans Methods: Synchronization and Observation
Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
Prostaglandin Extraction and Analysis in Caenorhabditis elegans
Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
De Novo Generation of Somatic Stem Cells by YAP/TAZ
Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4',6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved