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Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

DOI :

10.3791/55312-v

February 25th, 2017

February 25th, 2017

7,132 Views

1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

We describe sensitive, gel-based discontinuous assays to examine the kinetics of lagging-strand initiation using the replication proteins of bacteriophage T7.

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