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Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity

DOI :

10.3791/50695-v

August 19th, 2013

August 19th, 2013

19,025 Views

1Laboratory of Structural Biology, NIEHS, National Institutes of Health

Time courses for the glycosylase activity of 8-oxoguanine DNA glycosylase are biphasic exhibiting a burst of product formation and a linear steady-state phase. Utilizing quench-flow techniques, the burst and the steady-state rates can be measured, which correspond to excision of 8-oxoguanine and release of the glycosylase from the product DNA, respectively.

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Steady state

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