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A High-Throughput Enzyme-Coupled Activity Assay to Probe Small Molecule Interaction with the dNTPase SAMHD1

DOI :

10.3791/62503-v

8:17 min

April 16th, 2021

April 16th, 2021

1,788 Views

1Science for Life Laboratory, Department of Oncology-Pathology, Karolinska Institutet

SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase with critical roles in human health and disease. Here we present a versatile enzyme-coupled SAMHD1 activity assay, deployed in a 384-well microplate format, that allows for the evaluation of small molecules and nucleotide analogues as SAMHD1 substrates, activators, and inhibitors.

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High throughput

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