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Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

DOI :

10.3791/61436-v

8:07 min

May 19th, 2020

May 19th, 2020

2,900 Views

1Department of Biological Sciences, University of North Carolina at Charlotte

This protocol describes a method for culturing primary hippocampal neurons from embryonic mouse brain. The expression of major histocompatibility complex class I on the extracellular surface of cultured neurons is then assessed by flow cytometric analysis.

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Flow Cytometry

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