Name: chromatographic fingerprinting by template matching for data collected by comprehensive two-dimensional gas chromatography
Uploaded: 2020-09-02T14:00:00.000+00:00
Duration: 10 min 14 s
Description: Watch this Scientific Journal Video about Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography at JoVE.com

The research was supported by Progetto Ager &#8722; Fondazioni in rete per la ricerca agroalimentare. Project acronym Violin - Valorization of Italian olive products through innovative analytical tools (https://olivoeolio.progettoager.it/index.php/i-progetti-olio-e-olivo/violin-valorization-of-italian-olive-products-through-innovative-analytical-tools/violin-il-progetto). GC Image software is available for a free trial for readers who wish to demonstrate and test the protocol.

1. Importing raw data
NOTE: This creates a two-dimensional raster array for visualization and processing.
<ol>
	<li>Launch the image software.</li>
	<li>Select File | Import; navigate to and choose the raw data file acquired by GC&#215;GC-TOF MS system named &#8220;VIOLIN 101.lsc&#8221; (Supplementary File 1); then, click Open. The chromatogram opens in this software. 
	NOTE: Raw data file format depends of the instrument manufacturer. The software imports a variety of file formats listed in the user&#39;s guide.</li>
	<li>In the Import dialog, set the Modulation Period (PM) to 3.5 s; then, click OK. 
	NOTE: Some acquisition software may not record the modulation period.</li>
	<li>Select File | Save Image As; navigate to the desired folder; enter the name &#8220;Oil 1 RAW.gci&#8221; (Supplementary File 2); then, click Save.</li>
</ol>
2. Shifting the modulation phase
NOTE: This puts all peaks in each modulation cycle into the same image column, including the peaks that wrap around the end of the modulation period into the void time of the next modulation period20.
<ol>
	<li>Select Processing | Shift Phase.</li>
	<li>In the Shift Phase dialog, set the Shift Amount to -0.8 s; then, click OK.</li>
</ol>
3. Baseline correction21
<ol>
	<li>Select Graphic | Draw Rectangle.</li>
	<li>Click-and-drag to draw a rectangle in the image where no peaks are detected.</li>
	<li>Select Tools | Visualize Data; note the mean and standard deviation of the detector signal, here, 21.850 &#177; 1.455 SD unit-less digital number (DN); then, close the tool.</li>
	<li>Select Processing | Correct Baseline.</li>
</ol>
4. Coloring the chromatographic image using a value map and color map20
<ol>
	<li>Select View | Colorize.</li>
	<li>In the Colorize dialog, select the Import/Export tab; select the #AAAA (Supplementary File 3) custom color map provided as supplementary material; then, click Import.</li>
	<li>On the Value Mapping controls, set the value range to the minimum and maximum values; then, click OK.</li>
</ol>
5. 2D peaks (i.e., blobs) detection for analytes18
<ol>
	<li>Select Processing | Detect Blobs with the default settings; then, observe that some peaks are split and there are spurious detections.</li>
	<li>Select Configure | Settings | Blob Detection; then set Smoothing to 0.1 for the first dimension and 2.0 for the second dimension and set Minimum Volume (i.e., threshold for the summed values) to 1.00 E6; then, click OK.</li>
	<li>Select Processing | Detect Blobs with the new settings; then, observe the improvements.</li>
</ol>
6. 2D peaks filtration
NOTE: This is done to automatically remove meaningless detections due to column bleeds along the 1D and strikes or tailings along the 2D.
<ol>
	<li>Select Processing | Interactive Blob Detection.</li>
	<li>Note the blob detection settings; then, click Detect.</li>
	<li>In the Advanced Filter builder, click Add; then, in the New Constraint dialog, select Retention II; then, click OK.</li>
	<li>In the Constraint sliders, set the minimum and maximum 2D retention times for the filter to reduce the number of false peaks without losing true peaks.</li>
	<li>Click Apply; then, click Yes to save to the detection settings with the new filter. 
	NOTE: More advanced tools may be required to deal with particular detection problems, such as ion-peak detection or deconvolution for co-elutions19.</li>
</ol>
7. Linear retention indices calibration
NOTE: Perform this step22 (IT) for the specific retention times across the set of retention index (RI) standards (typically n-alkanes).
<ol>
	<li>Select Configure | RI Table | Retention Index (Col I).</li>
	<li>On the RI Table Configuration dialog, click Import; then, select the RI calibration file (in CSV format with name, retention time, and retention index) named &#8220;LRI table.csv&#8221; &#8211; (Supplementary File 4).</li>
	<li>Select File | Save Image A. Navigate to the desired folder; enter the name &#8220;Oil 1 LRI CALIBRATED.gci&#8221; (Supplementary File 5); then, click Save.</li>
</ol>
8. Searching for the peak spectra in the NIST17 MS library23
<ol>
	<li>Select Configure | Settings | Search Library.</li>
	<li>In the Search Library dialog, set Type of Spectrum to Peak MS, Intensity Threshold to 100, NIST Search Type to Simple (Similarity), NIST RI Column Type to Standard Polar, and NIST RI Tolerance to 10; then, click OK. NIST MS Search offers many other settings that are set to the defaults here.</li>
	<li>Select Processing | Search Library for All Blobs.</li>
</ol>
9. Review and correct analyte identifications
<ol>
	<li>On the tool palette, set the cursor mode to Blob | Select Blobs.</li>
	<li>In the Image view, right-click on the desired peak.</li>
	<li>On the Blob Properties dialog, inspect blob properties; then, click Hit List.</li>
	<li>Inspect the hit list; then, if the identification is incorrect, select the checkmark beside the correct identification.</li>
	<li>In the Blob Properties dialog, enter the Group Name to designate chemical class and any other desired metadata; then, click OK.</li>
	<li>Select File | Save Image As; navigate to the desired folder; enter the name &#8220;Oil 1 COLORIZED for Template construction.gci&#8221; (Supplementary File 6); then, click Save. 
	NOTE: This file is included in the supplemental archive, which can be opened for step 10.</li>
</ol>
10. Create a template with targeted peaks15
<ol>
	<li>In the Image view (still in Select Blobs mode from step 9.1), select the desired peaks with a click on the first peak and CTRL + click on the additional peaks.</li>
	<li>On the tool palette, click the Add to Template button.</li>
	<li>When the template is complete, select File | Save Template; specify the folder and file name; then, click Save.</li>
	<li>Select File | Close Image. 
	NOTE: At this point, these instructions continue with the template created to include the desired target peaks, available as &#8220;Targeted tamplate.bt&#8221; (Supplementary File 7).</li>
</ol>
11. Match and apply the template
NOTE: Matching recognizes the template pattern in the detected peaks a new chromatogram. Applying the matching sets identifications and other metadata in the new chromatogram from the template.
<ol>
	<li>Select File | Open Image; navigate to and select the &#8220;Oil 2 COLORIZED.gci&#8221; (Supplementary File 8) chromatogram file (which is pre-processed); then, click Open.</li>
	<li>On the tool palette, set the cursor mode to Template | Select Objects.</li>
	<li>Select Template | Load Template.</li>
	<li>In the Load Template dialog, click Browse; navigate to and select the targeted peaks template &#8220;Targeted template.bt&#8221; (Supplementary File 7); then, click Open.</li>
	<li>In the Load Template dialog, click Load, and then Dismiss.</li>
	<li>In the Image view, right-click on a template peak; then, inspect its object properties, including the qCLIC and reference MS.</li>
	<li>Select Template | Interactive Match and Transform Template.</li>
	<li>In the Interactive Match interface, click Match All; then, review the matching results both in the table and in the image, in which each template peak is marked with unfilled circles and, if a match is made, there is a link to a filled circle for the detected peak.</li>
	<li>Edit the matches as desired; when satisfied, click Apply to transfer metadata from the template to the chromatogram. 
	NOTE: Matching constraints, such as the qCLIC, help match the correct pattern among the detected peaks of the new chromatogram. Constraint parameters include the type of MS signature used as template reference (peak MS or blob MS) and the threshold values for spectral similarity (Direct Match Factor (DMF) and Reverse Match Factor (RMF)). Here, parameters are set based on previous studies13,14 to limit false negative matches: peak MS and DMF and RMF similarity threshold 700.</li>
</ol>
12. Transform the template for substantially different chromatography
NOTE: This step is not necessary unless chromatographic conditions vary substantially causing the template to be misaligned with a new chromatogram, such as can be the case over long-term studies or after a new column is installed. In such cases, the template can be geometrically transformed in the chromatographic retention-times plane to better fit the new chromatogram12,13. In this example, the peak patterns of the template and chromatogram are similar, but differ in the retention-times geometry, such as would be seen for different chromatographic conditions.
<ol>
	<li>Repeat steps 11.2&#8211;11.5, except navigate to, select, and load Targeted template 2.bt (Supplementary File 9).</li>
	<li>Select Template | Interactive Match Template; then, click Edit Transform.</li>
	<li>In the Transform Template interface, vary the 1D and 2D scales, translations, and shears to better align the template with the detected peaks; then, click Transform Template.</li>
	<li>With the transformed template, click Edit Match; then, repeat steps 11.8&#8211;11.9.</li>
</ol>
13. Perform combined untargeted and targeted analysis across a set of chromatograms
NOTE: A combined untargeted and targeted (UT) template, also referred to as feature template24,25, when matched to each of a set of chromatograms, establishes correspondences between untargeted and targeted analytes, then consistent cross-sample features are extracted for pattern recognition.
<ol>
	<li>Perform pre-processing (steps 1&#8211;6) and UT template matching (steps 11.1&#8211;11.9) for all chromatograms in the set (i.e., 2D chromatograms of oils). Alternatively, automate this step with project software or similar software, not described here.</li>
	<li>Launch the Investigator software.</li>
	<li>Select File | Open analysis; then, select, and open &#8220;Feature Jove su 70 eV.gca&#8221; (Supplementary File 10).</li>
	<li>Click OK to open and examine the results.</li>
	<li>Click on the Compounds tab to review metric values and statistics for specific analytes (i.e., targeted analytes with associated chemical names) or untargeted analytes with (#) identifiers aligned across all chromatograms, then perform the steps below.
	<ol>
		<li>Click on the Attributes tab to review values and statistics for specific metrics across chromatograms.</li>
		<li>Click on the Summary tab to review the summary statistics for both compounds and features. If the chromatograms are from different classes, as in this case oils produced from olives harvested in two different regions of Italy, then the Summary tab lists Fisher ratio statistics (F and FDR), which provide insights into features for discriminating between classes.</li>
		<li>View various charts on all tabs and, if desired, perform Principal Component Analysis (PCA) on the Attributes tab.</li>
	</ol>
	</li>
</ol>
14. Modify the UT template for parallel MS analysis
NOTE: The analysis was performed with both 70 eV and 12 eV (i.e., high and low) electron ionization energies26,27.
<ol>
	<li>Open one of the 12 eV chromatograms, e.g., &#8220;Oil 1 12 eV RAW.gci&#8221; (Supplementary File 11), perform pre-processing (steps 1&#8211;6) and load the UT template &#8220;UT template 70 relaxed.bt&#8221; (Supplementary File 12) as described in steps 11.1&#8211;11.6. Files are provided as supplementary material.</li>
	<li>If necessary, adjust the template to fit the detected 12 eV peaks as described in step 12. Here, there is no significant misalignment because the tandem signals are multiplexed. However, it should be noted that because the different ionization settings produce different fragmentations, it is necessary to relax constraints for the qCLIC constraints on DMF and RMF spectral similarity (not demonstrated here).</li>
	<li>Select File | Save Template; specify the folder and file name, e.g., &#8220;UT template 12.bt&#8221; (Supplementary File 13); then, click Save.</li>
</ol>
15. Perform combined untargeted and targeted analysis across 12 eV chromatograms
<ol>
	<li>Select File | Open analysis; then select and open &#8220;Feature Jove su 12 eV.gca&#8221; - Supplementary File 14 file provided.</li>
	<li>Click OK to open and examine results.</li>
	<li>Click on the Compounds tab to review metric values, refer to 12 eV responses and statistics for specific analytes (i.e., targeted analytes with associated chemical names) or untargeted analytes with (#) identifiers aligned across all chromatograms, then perform the steps below.
	<ol>
		<li>Click on the Attributes tab to review values and statistics for specific metrics across chromatograms.</li>
		<li>Click on the Summary tab to review the summary statistics for both compounds and features at 12 eV. If the chromatograms are from different classes, as in this case oils produced from olives harvested in two different regions of Italy, then the Summary tab lists Fisher ratio statistics (F and FDR), which provide insights into features for discriminating between classes.</li>
		<li>View the various charts available on all the tabs and, if desired, perform Principal Component Analysis (PCA) on the Attributes tab.</li>
	</ol>
	</li>
</ol>

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E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reliable+peak+selection+for+multisample+analysis+with+comprehensive+two-dimensional+chromatography.">Reliable peak selection for multisample analysis with comprehensive two-dimensional chromatography.</a> Analytical Chemistry. 85 (10), 4974-4981 (2013).</li><li>Markes International. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Select-EV:+The+next+Generation+of+Ion+Source+Technology.">Select-EV: The next Generation of Ion Source Technology.</a> Technical Note. , (2016).</li><li>Tao, Q., Reichenbach, S. E., Heble, C., Wu, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+investigator+tools+for+finding+unique+and+common+components+in+multiple+samples+with+comprehensive+two-dimensional+chromatography.">New investigator tools for finding unique and common components in multiple samples with comprehensive two-dimensional chromatography.</a> Chromatography Today. , (2018).</li><li>Seeley, J. V., Seeley, S. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multidimensional+gas+chromatography:+fundamental+advances+and+new+applications.">Multidimensional gas chromatography: fundamental advances and new applications.</a> Analytical Chemistry. 85 (2), 557-578 (2013).</li><li>Tranchida, P. Q., Aloisi, I., Giocastro, B., Mondello, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+state+of+comprehensive+two-dimensional+gas+chromatography-mass+spectrometry+with+focus+on+processes+of+ionization.">Current state of comprehensive two-dimensional gas chromatography-mass spectrometry with focus on processes of ionization.</a> Trends in Analytical Chemistry. 105, 360-366 (2018).</li></ol>

Prof. Stephen E. Reichenbach and Dr. Qingping Tao have financial interests in GC Image, LLC. Dr. Daniela Peroni is an employee of SRA Instruments, a distributor of GC Image in Italy and France. Dr. Federico Stilo, Prof. Chiara Cordero, and Prof. Carlo Bicchi declare no conflicts of interest.

Visualization of GC&#215;GC-TOF MS data is a fundamental step for an appropriate understanding of the results achieved by comprehensive two-dimensional separations. Image plots with customized colorization allows analysts to appreciate detector response differences and thus the differential distribution of sample components. This visual approach completely changes the analysts&#39; perspective on the interpretation and elaboration of chromatograms. This first step, once understood and confidently used by chromatographers, opens a new perspective in further processing.
Another fundamental aspect of data processing is the accessibility to the full data matrix (i.e., MS spectral data and responses) for all sample points, each of which corresponds to a single detector event. In this respect 2D peaks integration, so that the collection of detector events corresponding to a single analyte represent a critical step. In the current protocol, 2D peaks detection is based on the watershed algorithm18 with some adaptations included to improve detection sensitivity in case of partial co-eluting compounds. To make this process more specific, deconvolution must be done, and more sophisticated procedures adopted. This is possible by performing an ion peak detection for MS data; the algorithm processes the data array and isolates the response from single analytes based on spectral profiles19,31.
An important yet critical step of the protocol, and of any GC&#215;GC-MS data interpretation process, relates to analytes identification. This procedure, proposed in steps 8 and 9, in absence of a confirmatory analysis with authentic standards, must be carefully conducted by the analyst. Automated actions are available in any commercial software; they include MS spectral signature similarity evaluation against the collected reference spectra (i.e., spectral libraries) and evaluation of characteristic ratios among qualifier/quantifier ions. However, additional confirmatory criteria are needed to disambiguate identification of isomers. The protocol proposes the adoption of linear retention indexes to prioritize the list of candidates; the limit here relates to the availability of retention data and its consistency.
The main characteristic that makes this approach unique is template matching12,13,15,29. Template matching enables 2D pattern recognition in a very effective, specific, and intuitive way. It can be set, in terms of sensitivity and specificity, by applying customized threshold values and/or constraint functions while the analyst can supervise the procedure by actively interacting with transform function parameters. The peculiarity of this process relies on the possibility to cross-align targeted and untargeted peaks information between samples of a uniform batch but also between samples acquired with the same nominal conditions despite medium-to-severe misalignment. Advantages of this operation relate to the possibility to preserve all targeted analytes identifications, which is a time-consuming task for the analyst, and all metadata saved for targeted and untargeted peaks from previous elaboration sessions.
Template matching is also very effective in terms of computational time; low-resolution MS data files consists of about 1&#8211;2 Gb of packed data while high-resolution MS analyses may reach 10&#8211;15 Gb per single analytical run. Template matching does not process the full data matrix every time but, at first, performs retention-time alignment between chromatograms using template peaks then, processes candidate peaks within the search window for their similarity match with reference in the template. In case of severe misalignment, the most challenging situation, global second-order polynomial transforms performed better than local methods while reducing computational time13.
For the GC&#215;GC technique to spread widely beyond academia and research laboratories, data processing tools have to facilitate basic operations for visualization and chromatograms inspection; identification of analytes should offer the possibility to adopt standardized algorithms and procedures (e.g., NIST search algorithm and IT calibration); and cross-comparative analysis should be intuitive, effective and supported by interactive tools. The proposed approach addresses these needs while offering advanced options and tools to deal with complex situations such as analytes co-elution, multiple analytes calibration, group-type analysis, and parallel detection alignment.
The referenced literature well covers many possible scenarios where GC&#215;GC and, more generally, comprehensive two-dimensional chromatography, offer unique solutions and reliable results that cannot be achieved by 1D-chromatography in single run analysis.5,32,33 Although GC&#215;GC is the most powerful tool that increases separation capacity and sensitivity, there are always limitations to separation power, sensitivity, and other systemic capacities. As these systemic limits are approached, data analysis becomes progressively more difficult. Therefore, research and development must continue to improve the analytical tools at our disposal.

Comprehensive two-dimensional gas chromatography combined with the time-of-flight mass spectrometric detection (GC&#215;GC-TOF MS) is nowadays the most informative analytical approach for the chemical characterization of complex samples1,2,3,4,5. In GC&#215;GC, columns are serially connected and interfaced by a modulator (e.g., a thermal or valve-based focusing interface) that traps eluting components from the first dimension (1D) column before their re-injection into the second dimension (2D) column. This operation is done within a fixed modulation time-period (PM), generally ranging between 0.5&#8211;8 s. By thermal modulation, the process includes cryo-trapping and focusing of the eluting band with some benefits for the overall separation power.
Although GC&#215;GC is a two-dimensional separation technique, the process produces sequential data values. The detector analog-to-digital (A/D) converter obtains the chromatographic signal output at a certain frequency. Then, data is stored in specific proprietary formats which not only contains the digitalized data but related metadata (information about the data) as well. The A/D converter employed in GC&#215;GC systems helps in mapping the intensity of the chromatographic signal to a digital number (DN) as a function of time in the two analytical dimensions. Single-channel detectors (e.g., flame ionization detector (FID), electron capture detector (ECD), sulfur chemiluminescence detector (SCD), etc.) produce single values per sampling time, whereas multichannel detectors (e.g., mass spectrometric detector (MS)) produce multiple values (typically, over a spectral range) per sampling time along the analytical run.
To visualize 2D data, elaboration starts with rasterization of a single modulation period (or cycle) data values as a column of pixels (picture elements corresponding to detector events). Along the ordinate (Y-axis, bottom-to-top) the 2D separation time is visualized. Pixel columns are sequentially processed so that the abscissa (X-axis, left-to-right) reports 1D separation time. This ordering presents the 2D data in a right-handed Cartesian coordinate system, with the 1D retention ordinal as the first index into the array.
Data processing of 2D chromatograms gives access to a higher level of information than raw data, enabling 2D peak detection, peak identification, extraction of response data for quantitative analysis, and cross-comparative analysis.
The 2D peak patterns can be treated as the sample&#8217;s unique fingerprint and detected compounds as minutiae features for effective cross-comparative analysis. This approach, known as template-based fingerprinting6,7, was inspired by biometric fingerprinting6. Automatic biometric fingerprint verification systems, in fact, rely on unique fingertip characteristics: ridge bifurcations and endings, localized and extracted from inked impressions or detailed images. These characteristics, named minutiae features, are then cross-matched with available stored templates8,9.
As mentioned above, every GC&#215;GC separation pattern is composed of 2D peaks rationally distributed over a two-dimensional plane. Each peak corresponds to a single analyte, has its informative potential, and can be treated as a single feature for comparative pattern analysis.
Here, we present an effective approach for chemical fingerprinting by GC&#215;GC-TOF MS featuring tandem ionization. The goal is to comprehensively and quantitatively catalog features from a set of chromatograms.
Compared to existing commercial software or in-house routines10,11 that employ a peak-features approach, template-based fingerprinting is characterized by high specificity, efficiency, and limited computational time. In addition, it has an intrinsic flexibility that enables the cross-alignment of minutia features (i.e., 2D peaks) between severely misaligned chromatograms as those acquired by different instrumentation or in long-time frame studies12,13,14.
The basic operations of the proposed method are described briefly to guide the reader to a good understanding of the 2D pattern complexity and information power. Then, by exploring the instrument output data matrix, chemical identification is performed and known targeted analytes located over the two-dimensional space. The template of targeted peaks is then built and applied to a series of chromatograms acquired within the same analytical batch. Metadata related to retention times, spectral signatures, and responses (absolute and relative) are extracted from re-aligned patterns of targeted peaks and adopted to reveal compositional differences in the sample set.
As an additional, unique step of the process, a combined untargeted and targeted (UT) fingerprinting is also performed on pre-targeted chromatograms to extend the fingerprinting potential to both known and unknown analytes. The process produces a UT template for a truly comprehensive comparative analysis that can be largely automated.
As a final step, the method performs the cross-alignment of features in two parallel detector signals produced with high and low electron ionization energies (70 and 12 eV).
The protocol is quite flexible in supporting analyses of a single chromatogram or a set of chromatograms and with variable chromatography and/or multiple detectors. Here, the protocol is demonstrated with a commercially available GC&#215;GC Software suite (see Table of materials) combined to a MS library and search software (see Table of Materials). Some of the necessary tools are available in other software and similar tools could be implemented independently from descriptions in the literature by Reichenbach and co-workers15,16,17,18,19. Raw data for the demonstration is derived from a research study on extra-virgin olive (EVO) oil conducted in the authors&#8217; laboratory14. In particular, the volatile fraction (i.e., volatilome) of Italian EVO oils is sampled by headspace solid phase microextraction (HS-SPME) and analyzed by GC&#215;GC-TOF MS to capture diagnostic fingerprints for quality and sensory qualification of samples. Details on samples, sampling conditions, and analytical set-up are provided in the Table of Materials.
Steps 1&#8211;6 describe pre-processing of the chromatograms. Steps 7&#8211;9 describe processing and analysis of individual chromatograms. Steps 10&#8211;12 describe template creation and matching, which are the basis for cross-sample analysis. Steps 13&#8211;16 describe applying the protocol across a set of chromatograms, with steps 14&#8211;16 for UT analysis.

<table><tbody><tr><td>&lt;sup&gt;1&lt;/sup&gt;D SolGel-Wax column (100% polyethylene glycol; 30 m &amp;times; 0.25 mm dc &amp;times; 0.25 &amp;mu;m df). Carrier gas helium at a constant nominal flow of 1.3 mL/min.</td><td>Trajan SGE Analytical Science, Ringwood, Australia</td><td>PN 054796</td><td>Carrier gas helium at a constant nominal flow of 1.3 mL/min. Oven temperature programming set as follows: 40&amp;deg;C (2 min) to 240&amp;deg;C (10 min) at 3.5&amp;deg;C/min.</td></tr><tr><td>&lt;sup&gt;2&lt;/sup&gt;D OV1701 column (86% polydimethylsiloxane, 7% phenyl, 7% cyanopropyl; 1 m &amp;times; 0.1 mm dc &amp;times; 0.10 &amp;mu;m df) from .</td><td>Mega, Legnano, Milan, Italy</td><td>PN MEGA-1701</td><td></td></tr><tr><td>Automated system for sample preparation: SPR Autosampler for GC</td><td>SepSolve-Analytical, Llantrisant, UK</td><td></td><td></td></tr><tr><td>Extra Virgin Olive oils: Sicily and Tuscany, Italy</td><td>Project VIOLIN (Ager - Fondazioni in rete per la ricerca agroalimentare)</td><td></td><td>Samples (n=10) were collected during the production year 2018 within the &quot;Violin&quot; project sampling campaign. Oils were submitted to HS-SPME to sample volatiles according to a reference protocol validated in a previous study of Stilo et al.14</td></tr><tr><td>Gas chromatograph: Model 7890B GC</td><td>Agilent Technologies Wilmington DE, USA</td><td></td><td></td></tr><tr><td>GC Image GC&amp;times;GC edition V 2.9</td><td>GC Image LLC, Lincoln, Nebraska</td><td></td><td>https://www.gcimage.com/gcxgc/trial.html</td></tr><tr><td>Image processing software</td><td>GC Image LLC, Lincoln, Nebraska</td><td></td><td>https://www.gcimage.com/gcxgc/trial.html</td></tr><tr><td>Mass spectrometer: BenchTOF-Select</td><td>Markes International Llantrisant, UK</td><td></td><td></td></tr><tr><td>Methyl-2-octynoate (CAS 111-12-6)</td><td>Merck-Millipore/Supelco</td><td>PN: 68982</td><td></td></tr><tr><td>Modulator controller: Optimode v2.0</td><td>SRA Intruments, Cernusco sul Naviglio, Milan, Italy</td><td></td><td></td></tr><tr><td>Modulator: KT 2004 loop type</td><td>Zoex Corporation Houston, TX, USA</td><td></td><td></td></tr><tr><td>MS library and search software: NIST Library V 2017, Software V 2.3</td><td>National Institute of Standards and Technology (NIST), Gaithersburg MD</td><td></td><td>https://www.nist.gov/srd/nist-standard-reference-database-1a-v17</td></tr><tr><td>&lt;em&gt;n&lt;/em&gt;-alkanes C8-C40 for retention indexing</td><td>Merck-Millipore/Supelco</td><td>PN: 40147-U</td><td></td></tr><tr><td>&lt;em&gt;n&lt;/em&gt;-hexane (CAS 110-54-3) gas chromatography MS SupraSolv</td><td>Merck-Millipore/Supelco</td><td>PN: 100795</td><td></td></tr><tr><td>Solid Phase Microextraction fiber</td><td>Merck-Millipore/Supelco</td><td>PN 57914-U</td><td></td></tr><tr><td>&amp;alpha;- /&amp;beta;-thujone (CAS 546-80-5)</td><td>Merck-Millipore/Sigma Aldrich</td><td>PN: 04314</td><td></td></tr></tbody></table>

chromatographic fingerprinting by template matching for data collected by comprehensive two-dimensional gas chromatography

Data processing and evaluation are critical steps of comprehensive two-dimensional gas chromatography (GCxGC), particularly when coupled to mass spectrometry. The rich information encrypted in the data may be highly valuable but difficult to access efficiently. Data density and complexity can lead to long elaboration times and require laborious, analyst-dependent procedures. Effective yet accessible data processing tools, therefore, are key to enabling the spread and acceptance of this advanced multidimensional technique in laboratories for daily use. The data analysis protocol presented in this work uses chromatographic fingerprinting and template matching to achieve the goal of highly automated deconstruction of complex two-dimensional chromatograms into individual chemical features for advanced recognition of informative patterns within individual chromatograms and across sets of chromatograms. The protocol delivers high consistency and reliability with little intervention. At the same time, analyst supervision is possible in a variety of settings and constraint functions that can be customized to provide flexibility and capacity to adapt to different needs and goals. Template matching is shown here to be a powerful approach to explore extra-virgin olive oil volatilome. Cross-alignment of peaks is performed not only for known targets, but also for untargeted compounds, which significantly increases the characterization power for a wide range of applications. Examples are presented to evidence the performance for the classification and comparison of chromatographic patterns from sample sets analyzed under similar conditions.

GC&#215;GC-TOF MS patterns of high-quality extra-virgin olive oil volatilome exhibit about 500 2D peaks above a signal-to-noise ratio (SNR) threshold of 100. Such a threshold was defined by previous investigations on food volatiles14,27 as the minimum relative signal over threshold to obtain reliable spectra for cross-comparative analysis. Components are distributed over the chromatographic space according to their relative retention in the two chromatographic dimensions, and specifically based on their volatility/polarity in the 1D and volatility in the 2D. Here, column combination is polar &#215; semi-polar (i.e., Carbowax 20M &#215; OV1701).
The 2D pattern shows a high degree of order. Relative retention patterns for homologous series and classes are shown in Figure 1A with annotations (graphics for groups and bubbles for peaks) for linear saturated hydrocarbons (black), unsaturated hydrocarbons (yellow), linear saturated aldehydes (blue), mono-unsaturated aldehydes (red), polyunsaturated aldehydes (salmon), primary alcohols (green), and short-chain fatty acids (cyano).
Detected 2D peaks can then be identified by comparing the average MS spectrum extracted from the entire 2D peak (blob spectrum) or from the largest spectrum (apex spectrum). Figure 2 illustrates the output of the apex spectrum search for blob 5 and returns a high similarity match (first 10 hits) for (E)-2-hexenal. Databases explored are those pre-selected by the analyst in step 8 of the method.
The identification is validated by active retention indexing. The experimental IT value was calculated for the 2D peaks, so that at this stage the library search prioritizes results with coherent values of tabulated IT. Tolerance windows can be customized based on analyst experience, reliability of reference database values according to stationary phase, and analytical conditions applied. New tools for smart calibration of linear retention indices without experimental calibration with n-alkanes, have been recently developed and discussed in a study by Reichenbach et al19.
The collection of identified 2D peaks (i.e., targeted peaks) can be adopted to build a template of targeted peaks to promptly establish reliable correspondences between the same compound across all sample chromatograms. The collection of targeted template peaks is visualized in Figure 1B. Red circles correspond to the 196 targeted compounds, including two Internal Standards (IS) linked to template peaks with connection lines. IS are used for response normalization and connection lines help to visualize which of the included IS will be adopted to normalize each 2D peak/blob response.
In Figure 1B, filled circles indicate positive matches between template peak and the actual pattern while empty circles are for template peaks for which the correspondence was not verified. False negative matches can be limited by appropriate selection of threshold parameters, reference spectra and constraint functions13,14,18,19. For complex patterns with multiple co-elutions, ion peak detection functions that are based on spectral deconvolution are advisable and could be a valid option19. Template peak metadata are shown in the enlarged panel of Figure 1B for (E)-2-hexenal.
The specificity of template matching relies on the possibility to apply constraint functions that limit positive correspondence to those candidate peaks that, falling within the search window of the algorithm, have MS spectral similarity above a certain threshold. In this case, in step 11, similarity thresholds23 were set at 700 according to previous experiments aimed at defining optimal parameters limiting false negative matches14. Highlighted areas of the template peak properties in Figure 1B show the information about the reference MS spectrum string and the qCLIC constraint function (i.e., (Match(&#34;&#60;ms&#62;&#34;) &#62;= 700.0) and (RMatch(&#34;&#60;ms&#62;&#34;) &#62;= 700.0)).
By applying the template to all chromatograms of a set, one could encounter challenging situations as in the case of partial misalignment of patterns. This can be due to oven temperature inconsistencies, carrier gas flow/pressure instabilities, or because of a manual intervention on the system as in the case of column substitution or modulator loop-capillary replacement14,28. Figure 3 shows a situation of a partial misalignment between the targeted template and the actual chromatogram. For minimal misalignments, interactive template transforms (Figure 3, control panel) can reposition template peaks for a better fit. Once repositioned, the template can be matched to establish correspondences. In the example, the template (Figure 3, step 12) peaks correctly match with the actual 2D pattern. In case of severe misalignments, not discussed here, the repetition of match-transform-update actions can iteratively adapt the template peaks position to the actual peak pattern12,13,14.
Here, the targeted peaks (i.e., known analytes) provide about 40% of the chromatographic result (196 targeted peaks of about 500 detectable peaks on average). The other 60% of compounds, together with the information they bring, are not taken into consideration in targeted analysis. To make the investigation truly comprehensive, consistent cross-alignment of untargeted 2D peaks should also be established. The first application where template matching was extended to all detectable analytes dealt with the complex volatilome of roasted coffee7. This process is automated with a software (e.g., Investigator), shown here in steps 14&#8211;15.
In this process, pre-targeted images belonging to the sample set under study (20 samples) are used to define reliable peaks by cross-matching of all image patterns29. Subsequently, a composite chromatogram is built from which one can identify UT reliable peaks and peak regions (i.e., 2D peaks footprint) in the so-called feature template17.
For analyses acquired at 70 eV, the process determined 144 reliable peaks with relaxed reliability29, 76 of which belong to the targeted peaks list. Based on these 144 reliable peaks, the process aligns all chromatograms consistently with the average retention times of the reliable peaks and then combines them to create a composite chromatogram. Figure 4 shows a list of all samples labeled according to the production region of the oil (left) and the list of reliable peaks/blob volumes in each sample (right).
The untargeted feature template is composed of 2D peaks from analytes detected in the composite chromatogram, shown in Figure 5A, that are matched by the reliable-peaks template (n = 168 &#8211; red circles for targeted peaks and green circles for untargeted peaks). The mass spectra of the composite peaks, as well as their retention times, are recorded in the feature template as shown for (Z)-3-hexenol acetate in the enlarged area. Peak-regions are shown in Figure 5B as red colored graphics; they are instead defined by the outlines of all 2D peaks detected in the composite chromatogram (n = 3578).
When unsupervised pattern recognition by Principal Component Analysis is applied to targeted peaks distribution within the 20 analyzed samples, Sicilian and Tuscany oils cluster separately suggesting that pedo-climatic conditions and terroir impact the relative prevalence of volatiles. The results are shown in Figure 6A and the PCA results from the reliable peaks distribution are shown in Figure 6B. The two approaches cross-validate that oils from different geographical areas have different, while coherent, chemical signatures whether targeted or untargeted compounds, or both, are mapped.
Finally, the software enables prompt and effective re-alignment of patterns across parallel detection channels. In this application, the re-alignment is proposed for tandem ionization signals. The ion source of the MS multiplexes between two ionization energies (i.e., 70 and 12 eV) at an acquisition frequency of 50 Hz per channel30. The two resulting chromatographic patterns are closely aligned while spectral data (i.e., spectral signatures and responses)&#160;bring complementary information with different dynamic ranges of response26,27. The aligned patterns allow extracting features (2D peaks and peak-regions) with univocal IDs (i.e., chemical names for targeted peaks and unique numbering # for untargeted peaks and peak-regions).
Template matching allows effective cross-alignment. In this situation, there is not much misalignment, but MS constraints must be relaxed to allow matches for UT peaks. On the other hand, featured UT peak-regions, that have no MS constraints, are promptly matched without any false negative matches. Figure 5C shows an enlarged area of a 12 eV chromatogram where the feature template built from 70 eV data is matched. Reliable UT peaks are positively matched because of the lowered qCLIC constraints (e.g., DMF threshold at 600). To note, at 12 eV, there are fewer detected peaks due to the limited fragmentation induced by low ionization energy.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/61529/61529fig01.jpg" /> 
Figure 1: Bidimensional contour plot and targeted template.&#160;(A) Contour plot of the volatile fraction of an extra-virgin olive oil from Tuscany. Ordered patterns of homolog series and classes are highlighted with different colors and lines: linear saturated hydrocarbons (black line and 2D contours) unsaturated hydrocarbons (yellow), linear saturated aldehydes (blue) mono-unsaturated aldehydes (red), polyunsaturated aldehydes (salmon), primary alcohols (green) and short-chain fatty acids (cyano). (B) Overimposed targeted template of known analytes (red colored circles) with connection lines linking Internal Standards (ISs). Panels show 2D peak/blob properties metadata (Decanal) or Template peak properties. <a href="https://www.jove.com/files/ftp_upload/61529/61529fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/61529/61529fig02.jpg" /> 
Figure 2: Apex MS search.&#160;Output of the apex MS search for blob 5. List of the database entries with the highest similarity match and related metadata available from the library. <a href="https://www.jove.com/files/ftp_upload/61529/61529fig02large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/61529/61529fig03.jpg" /> 
Figure 3: Template realignment.&#160;Workflow illustrating the steps that allow re-alignment of the template by transformation. <a href="https://www.jove.com/files/ftp_upload/61529/61529fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/61529/61529fig04.jpg" /> 
Figure 4: GC Investigator interface.&#160;Investigator panel with all selected images labeled according to the production Region of the oil (left) and the list of reliable peaks/blob volumes in each sample (right). <a href="https://www.jove.com/files/ftp_upload/61529/61529fig04large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 5" class="xfigimg" src="/files/ftp_upload/61529/61529fig05.jpg" /> 
Figure 5: Targeted and UT template.&#160;(A) Reliable peaks as resulting from the automated processing in step 11; red circles correspond to known analytes while green circles are unknowns. In the superimposed panel, template object properties are shown for the (Z)-3-hexenal. (B) Enlarged area that shows the UT peaks (red and green circles) and peak-regions (red graphics) of the UT template matched on a sample oil acquired at 70 eV ionization energy. (C) UT template matched on a sample oil acquired at 12 eV ionization energy. <a href="https://www.jove.com/files/ftp_upload/61529/61529fig05large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 6" class="xfigimg" src="/files/ftp_upload/61529/61529fig06.jpg" /> 
Figure 6: PCA loading plots.&#160;They show the natural conformation of samples (oils from Tuscany and Sicily) as they result by (A) targeted peaks distribution or (B) UT peaks distribution. <a href="https://www.jove.com/files/ftp_upload/61529/61529fig06large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
Supplemental Files. <a href="https://www.jove.com/files/ftp_upload/61529/Supplemental_Files.zip" target="_blank">Please click here to download these files.</a>

Watch this Scientific Journal Video about Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography at JoVE.com

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

This protocol presents an approach to fingerprint and explore multi-dimensional data collected by comprehensive two-dimensional gas chromatography coupled to mass spectrometry. Dedicated pattern recognition algorithms (template matching) are applied to explore the chemical information encrypted in the extra-virgin olive oil volatile fraction (i.e., volatilome).

Data processing and evaluation are critical steps of comprehensive two-dimensional gas chromatography (GCxGC), particularly when coupled to mass ...

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Chemistry

4.7K Views.  Università degli Studi di Torino. This protocol presents an approach to fingerprint and explore multi-dimensional data collected by comprehensive two-dimensional gas chromatography coupled to mass spectrometry. Dedicated pattern recognition algorithms (template matching) are applied to explore the chemical information encrypted in the extra-virgin olive oil volatile fraction (i.e., volatilome).

Video: Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector

The direct liquid deposition of solution standards onto sorbent-filled thermal desorption tubes is used for the quantitative analysis of trace explosive vapor samples. The direct liquid deposition method yields a higher fidelity between the analysis of vapor samples and the analysis of solution standards than using separate injection methods for vapors and solutions, i.e., samples collected on vapor collection tubes and standards prepared in solution vials. Additionally, the method can account for instrumentation losses, which makes it ideal for minimizing variability and quantitative trace chemical detection. Gas chromatography with an electron capture detector is an instrumentation configuration sensitive to nitro-energetics, such as TNT and RDX, due to their relatively high electron affinity. However, vapor quantitation of these compounds is difficult without viable vapor standards. Thus, we eliminate the requirement for vapor standards by combining the sensitivity of the instrumentation with a direct liquid deposition protocol to analyze trace explosive vapor samples.

Trace explosive vapors of TNT and RDX collected on sorbent-filled thermal desorption tubes were analyzed using a programmed temperature desorption system coupled to GC with an electron capture detector. The instrumental analysis is combined with direct liquid deposition method to reduce sample variability and account for instrumentation drift and losses.

The direct liquid deposition of solution standards onto sorbent-filled thermal desorption tubes is used for the quantitative analysis of trace explosive ...

On-line Analysis of Nitrogen Containing Compounds in Complex Hydrocarbon Matrixes

The shift to heavy crude oils and the use of alternative fossil resources such as shale oil are a challenge for the petrochemical industry. The composition of heavy crude oils and shale oils varies substantially depending on the origin of the mixture. In particular they contain an increased amount of nitrogen containing compounds compared to the conventionally used sweet crude oils. As nitrogen compounds have an influence on the operation of thermal processes occurring in coker units and steam crackers, and as some species are considered as environmentally hazardous, a detailed analysis of the reactions involving nitrogen containing compounds under pyrolysis conditions provides valuable information. Therefore a novel method has been developed and validated with a feedstock containing a high nitrogen content, i.e., a shale oil. First, the feed was characterized offline by comprehensive two-dimensional gas chromatography (GC &#215; GC) coupled with a nitrogen chemiluminescence detector (NCD). In a second step the on-line analysis method was developed and tested on a steam cracking pilot plant by feeding pyridine dissolved in heptane. The former being a representative compound for one of the most abundant classes of compounds present in shale oil. The composition of the reactor effluent was determined via an in-house developed automated sampling system followed by immediate injection of the sample on a GC &#215; GC coupled with a time-of-flight mass spectrometer (TOF-MS), flame ionization detector (FID) and NCD. A novel method for quantitative analysis of nitrogen containing compounds using NCD and 2-chloropyridine as an internal standard has been developed and demonstrated.

A method combining comprehensive two-dimensional gas chromatography with nitrogen chemiluminescence detection has been developed and applied to on-line analysis of nitrogen containing compounds in a complex hydrocarbon matrix.

The shift to heavy crude oils and the use of alternative fossil resources such as shale oil are a challenge for the petrochemical industry. The ...

A Two-Step Pyrolysis-Gas Chromatography Method with Mass Spectrometric Detection for Identification of Tattoo Ink Ingredients and Counterfeit Products

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. In this method for two-step pyrolysis online coupled to gas chromatography mass spectrometry (py-GC-MS) volatile compounds are analyzed during a first desorption run. In the second run, the same dried sample is pyrolyzed for analysis of non-volatile compounds such as pigments and polymers. These can be identified by their specific decomposition patterns. Additionally, this method can be used to differentiate original from counterfeit inks. Easy screening methods for data evaluation using the average mass spectra and self-made pyrolysis libraries are applied to speed up substance identification. Using specialized evaluation software for pyrolysis GS-MS data, a fast and reliable comparison of the full chromatogram can be achieved. Since GC-MS is used as separation technique, the method is limited to volatile substances upon desorption and after pyrolysis of the sample. The method can be applied for quick substance screening in market control surveys since it requires no sample preparation steps.

This method for two-step pyrolysis online coupled to gas chromatography with mass spectrometric detection and data evaluation protocol can be used for multi-component analysis of tattoo inks and discrimination of counterfeit products.

Tattoo inks are complex mixtures of ingredients. Each of them possesses different chemical properties which have to be addressed upon chemical analysis. ...

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Two-dimensional gas chromatography coupled with time-of-flight mass spectrometry is a powerful tool for identifying and quantifying chemical components in complex mixtures. It is often used to analyze gasoline, jet fuel, diesel, bio-diesel and the organic fraction of bio-crude/bio-oil. In most of those analyses, the first dimension of separation is non-polar, followed by a polar separation. The aqueous fractions of bio-crude and other aqueous samples from biofuels production have been examined with similar column combinations. However, sample preparation techniques such as derivatization, solvent extraction, and solid-phase extraction were necessary prior to analysis. In this study, aqueous fractions obtained from the hydrothermal liquefaction of algae were characterized by two-dimensional gas chromatography coupled with time-of-flight mass spectrometry without prior sample preparation techniques using a polar separation in the first dimension followed by a non-polar separation in the second. Two-dimensional plots from this analysis were compared with those obtained from the more traditional column configuration. Results from qualitative characterization of the aqueous fractions of algal bio-crude are discussed in detail. The advantages of using a polar separation followed by a non-polar separation for characterization of organics in aqueous samples by two-dimensional gas chromatography coupled with time-of-flight mass spectrometry are highlighted.

A two-dimensional gas chromatography-time-of-flight mass spectrometry method is described for characterization of the aqueous fraction of bio-crude produced from hydrothermal liquefaction of algae. This protocol can also be employed to analyze the aqueous fraction of liquid products from fast pyrolysis, catalytic fast pyrolysis, catalytic deoxygenation and hydro-treating.

Two-dimensional gas chromatography coupled with time-of-flight mass spectrometry is a powerful tool for identifying and quantifying chemical components in ...

Bioengineering

Nitrogen Compound Characterization in Fuels by Multidimensional Gas Chromatography

Certain nitrogen-containing compounds can contribute to fuel instability during storage. Hence, detection and characterization of these compounds is crucial. There are significant challenges to overcome when measuring trace compounds in a complex matrix such as fuels. Background interferences and matrix effects can create limitations to routine analytical instrumentation, such as GC-MS. In order to facilitate specific and quantitative measurements of trace nitrogen compounds in fuels, a nitrogen-specific detector is ideal. In this method, a nitrogen chemiluminescence detector (NCD) is used to detect nitrogen compounds in fuels. NCD utilizes a nitrogen-specific reaction that does not involve the hydrocarbon background. Two-dimensional (GCxGC) gas chromatography is a powerful characterization technique as it provides superior separation capabilities to one-dimensional gas chromatography methods. When GCxGC is paired with a NCD, the problematic nitrogen compounds found in fuels can be extensively characterized without background interference. The method presented in this manuscript details the process for measuring different nitrogen-containing compound classes in fuels with little sample preparation. Overall, this GCxGC-NCD method has been shown to be a valuable tool to enhance the understanding of the chemical composition of nitrogen-containing compounds in fuels and their impact on fuel stability. The % RSD for this method is &#60;5% for intraday and &#60;10% for interday analyses; the LOD is 1.7 ppm and the LOQ is 5.5 ppm.

Here, we present a method utilizing two-dimensional gas chromatography and nitrogen chemiluminescence detection (GCxGC-NCD) to extensively characterize the different classes of nitrogen-containing compounds in diesel and jet fuels.

Certain nitrogen-containing compounds can contribute to fuel instability during storage. Hence, detection and characterization of these compounds is ...

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Gas Chromatography &#8211; Mass Spectrometry (GC-MS) is a frequently used technique for the analysis of numerous analytes of forensic interest, including controlled substances, ignitable liquids, and explosives. GC-MS can be coupled with Solid-Phase Microextraction (SPME), in which a fiber with a sorptive coating is placed into the headspace above a sample or immersed in a liquid sample. Analytes are sorbed onto the fiber which is then placed inside the heated GC inlet for desorption. Total Vaporization Solid-Phase Microextraction (TV-SPME) utilizes the same technique as immersion SPME but immerses the fiber into a completely vaporized sample extract. This complete vaporization results in a partition between only the vapor phase and the SPME fiber without interference from a liquid phase or any insoluble materials. Depending upon the boiling point of the solvent used, TV-SPME allows for large sample volumes (e.g., up to hundreds of microliters). On-fiber derivatization may also be performed using TV-SPME. TV-SPME has been used to analyze drugs and their metabolites in hair, urine, and saliva. This simple technique has also been applied to street drugs, lipids, fuel samples, post-blast explosive residues, and pollutants in water. This paper highlights the use of TV-SPME to identify illegal adulterants in very small samples (microliter quantities) of alcoholic beverages. Both gamma-hydroxybutyrate (GHB) and gamma-butyrolactone (GBL) were identified at levels that would be found in spiked drinks. Derivatization by a trimethylsilyl agent allowed for conversion of the aqueous matrix and GHB into their TMS derivatives. Overall, TV-SPME is quick, easy, and requires no sample preparation aside from placing the sample into a headspace vial.

Total Vaporization Solid Phase Microextraction (TV-SPME) completely vaporizes a liquid sample whilst analytes are sorbed onto a SPME fiber. This allows for partitioning of the analyte between only the solvent vapor and the SPME fiber coating.

Gas Chromatography &#8211; Mass Spectrometry (GC-MS) is a frequently used technique for the analysis of numerous analytes of forensic interest, including ...

Gas Chromatography (GC) with Flame-Ionization Detection

Source: Laboratory of Dr. B. Jill Venton - University of Virginia
Gas chromatography (GC) is used to separate and detect small molecular weight compounds in the gas phase. The sample is either a gas or a liquid that is vaporized in the injection port. Typically, the compounds analyzed are less than 1,000 Da, because it is difficult to vaporize larger compounds. GC is popular for environmental monitoring and industrial applications because it is very reliable and can be run nearly continuously. GC is typically used in applications where small, volatile molecules are detected and with non-aqueous solutions. Liquid chromatography is more popular for measurements in aqueous samples and can be used to study larger molecules, because the molecules do not need to vaporize. GC is favored for nonpolar molecules while LC is more common for separating polar analytes.
The mobile phase for gas chromatography is a carrier gas, typically helium because of its&#160;low molecular weight and being chemically inert. Pressure is applied and the mobile phase moves the analyte through the column. The separation is accomplished using a column coated with a stationary phase. Open tubular capillary columns are the most popular columns and have the stationary phase coated on the walls of the capillary. Stationary phases are often derivatives of polydimethylsiloxane, with 5&#8211;10% of the groups functionalized to tune the separation. Typical functional groups are phenyl, cyanopropyl, or trifluoropropyl groups. Capillary columns are usually 5&#8211;50 m&#160;long. Narrower columns have higher resolution but require higher pressures. Packed columns can also be used where the stationary phase is coated onto beads packed in the column. Packed columns are shorter, 1&#8211;5 m. Open tubular capillaries are generally preferred because they allow higher efficiencies, faster analyses, and have higher capacities.
Flame-ionization detection (FID) is a good general detector for organic compounds in GC that detects the amount of carbon in a sample. After the column, samples are burned in a hot, hydrogen-air flame. Carbon ions are produced by the combustion. While the overall efficiency of the process is low (only 1 in 105 carbon ions produce an ion in the flame) the total amount of ions is directly proportional to the amount of carbon in the sample. Electrodes are used to measure the current from the ions. FID is a destructive detector, as the entire sample is pyrolyzed. FID is unaffected by noncombustible gases and water.

Gas Chromatography GC with Flame-Ionization Detection

Source: Laboratory of Dr. B. Jill Venton - University of Virginia
Gas chromatography (GC) is used to separate and detect small molecular weight compounds ...

Education

JoVE Science Education

Analytical Chemistry

Gas Chromatography: Introduction

Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC,&#160; a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column. The column is packed or coated with a stationary phase, either a liquid or solid material. Compounds in the sample are separated based on their boiling points, and compounds with lower boiling points elute first. The elution order- the sequence in which the compound exits the column- is visible on a chromatogram, where each peak corresponds to a specific compound.
There are two main types of gas chromatography:
1. Gas-liquid chromatography (GLC): In GLC, the stationary phase is a nonvolatile, thermally stable, chemically inert liquid, while the mobile phase is a carrier gas, typically helium. This technique is commonly used to separate compounds based on their boiling points.
&#160; 2. Gas-solid chromatography (GSC): GSC uses solid particulates like diatomaceous earth as the stationary phase and a carrier gas like helium as the mobile phase. GSC is primarily used for permanent gases and small polar molecules not retained by gas-liquid columns due to severe tailing of elution peaks.

Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify ...

JoVE Core

Unit 1

Principles of Chromatography

KEY TERMS AND CONCEPTS

Gas Chromatography&ndash;Mass Spectrometry (GC&ndash;MS)

Gas chromatography&#8211;mass spectrometry (GC&#8211;MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating material features a high boiling point, low viscosity, and low polarity and functions as the stationary phase of the chromatograph. The analyte is injected into one end of the column and flushed through the column using an inert gas flow as the mobile phase. The boiling point of each compound and its interaction with the stationary phase determines the speed of movement through the column. Consequently, each compound in the mixture reaches the other end of the column at a different time. Eventually, the mass spectrometer connected to the column&#39;s end analyzes each compound separately.

Gas Chromatography&amp;#8211;Mass Spectrometry GC&amp;#8211;MS

Gas chromatography&#8211;mass spectrometry (GC&#8211;MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a ...

Principles of Mass Spectrometry

Gas Chromatography: Overview of Detectors

Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, which are displayed on a chromatogram. Detectors can be categorized into two main types: destructive and non-destructive.
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and electron capture detectors.
On the other hand, a destructive detector causes chemical changes in the analyte or completely consumes it during detection, making it impossible to recover the sample afterward. This often involves burning or chemically reacting the sample, making recovery impossible. Examples include flame ionization and nitrogen-phosphorus detectors, which combust the sample.
An ideal gas chromatography detector should be non-destructive and possess high sensitivity for detecting low analyte concentrations. It should exhibit responsiveness to all analytes or selectively respond to specific classes of analytes while maintaining a linear response across a wide concentration range. Linearity indicates that the detector&#39;s response is directly proportional to the concentration of the analytes, making it easier to quantify the amount present. Stability, reliability, and reproducibility are crucial, with the detector being insensitive to variations in flow rate and temperature. Achieving a short response time, independent of flow rate, enhances the sample throughput and reduces analysis time for large sample quantities. Furthermore, the detector should exhibit minimal interference from sample matrix components and compatibility with various analyte types. Lastly, robustness and durability are vital characteristics, ensuring the detector&#39;s reliability and resilience under the conditions encountered in gas chromatography analysis.
Gas chromatography analysis commonly utilizes thermal conductivity, flame ionization, mass spectrometer, thermionic, electrolytic conductivity, photoionization, FTIR, and electron capture detectors.
Flame ionization detectors offer a broader linear response range, albeit destroying the sample, and possess a higher detection limit compared to thermal conductivity detectors. Electron capture detectors exhibit excellent detection limits but have a relatively narrow linear range. Ultimately, the choice of the detector depends on the type of the sample under analysis and the detector&#39;s typical detection limit.

Detectors in gas chromatography (GC) help identify and quantify the components of a mixture by translating chemical properties into measurable signals, ...

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

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