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Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

7.2K Views

10:10 min

August 19th, 2020

August 19th, 2020

7,224 Views

0:04

Introduction

0:35

Mouse and Human White Adipose Tissue Fixation, Permeabilization, and Saturation

2:59

Mouse and Human White Adipose Tissue Staining

5:28

Mouse and Human White Adipose Tissue Clearing

6:17

3D Confocal Imaging

7:23

3D Adipose Tissue Image Quantitative Result Extraction

7:57

Results: Representative Adipose Tissue Labeling and Analysis

9:34

Conclusion

필기록

These protocol allows analysis of three-dimensional structure of Very large human on mouse adipose tissue ballet microscopy. Facilitating the linkage of pathological dysfunction with adipose tissue structural changes. This clearing process are ver

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Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.

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