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JoVE Journal

Medicine

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A Murine Model of Ischemic Retinal Injury Induced by Transient Bilateral Common Carotid Artery Occlusion

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05:20 min

November 12th, 2020

November 12th, 2020

7,258 Views

0:04

Introduction

1:00

Transient Bilateral Common Carotid Artery Occlusion (tBCCAO)

3:12

Results: Analysis of Eyelid Drooping, HIF-1α Stabilization, Reactive Gliosis, Visual Dysfunction, and Change in Retinal Thickness After tBCCAO

4:40

Conclusion

Transcript

The mouse model established with this protocol can be utilized to investigate the pathophysiology of ischemic retinopathies and for in vivo drug screening. The current method could reduce reliance on various surgical instruments and shorten operat

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Here, we describe a mouse model of retinal ischemia by transient bilateral common carotid artery occlusion using simple sutures and a clamp. This model can be useful for understanding the pathological mechanisms of retinal ischemia caused by cardiovascular abnormalities.

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