JoVE Logo
Faculty Resource Center

Sign In

Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis

DOI :

10.3791/61559-v

June 27th, 2020

June 27th, 2020

5,958 Views

1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine, University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University

Following viral infection, kidney harbors a relatively large number of CD8+ T cells and offers an opportunity to study non-mucosal TRM cells. Here, we describe a protocol to isolate mouse kidney lymphocytes for flow cytometry analysis.

Tags

Isolation

-- Views

Related Videos

article

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

article

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

article

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

article

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

article

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

article

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

article

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

article

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

article

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells

article

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved