Live-cell Imaging of Single-Cell Arrays (LISCA) - a Versatile Technique to Quantify Cellular Kinetics
3.6K Views
•
10:24 min
•
March 18th, 2021
DOI :
March 18th, 2021
•Explore More Videos
Chapters in this video
0:04
Introduction
0:30
Microstructured Single Cell Array Fabrication
3:16
Cell Seeding
4:21
Perfusion System
5:13
Time-Lapse Microscopy
6:44
Fluorescent Marker mRNA Transfection
7:34
Image Analysis and Fluorescence Readout
8:29
Results: Representative Image Analysis and Fluorescence Readout
9:34
Conclusion
Related Videos
10:26
Live-cell Imaging of Migrating Cells Expressing Fluorescently-tagged Proteins in a Three-dimensional Matrix
13.1K Views
07:59
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
7.6K Views
08:29
Long-term Live-cell Imaging to Assess Cell Fate in Response to Paclitaxel
9.8K Views
12:06
Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
8.5K Views
09:09
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
8.5K Views
09:13
Multiplexed Live-Cell Imaging for Drug Responses in Patient-Derived Organoid Models of Cancer
1.0K Views
06:00
Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
10.9K Views
10:12
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
8.0K Views
10:55
Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
8.6K Views
09:04
Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
9.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved