July 10th, 2021
•The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.
Tags
Related Videos
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
Human Blastocyst Biopsy and Vitrification
Minimum Volume Vitrification of Immature Feline Oocytes
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Accurate Follicle Enumeration in Adult Mouse Ovaries
Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved