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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

DOI :

10.3791/62272-v

July 10th, 2021

July 10th, 2021

4,469 Views

1Department of Veterinary Medicine, University of Sassari

The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.

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