Selection and Isolation of the Follicles and Retrieval of the COCs
4:31
Long in Vitro Culture of the Oocytes (long IVCO
7:15
Results: Meiotic Progression of Oocytes after long IVCO
8:52
Conclusion
Transcript
In this video protocol, we demonstrate in detail, a culture system for growing bovine oocytes. The system supports the oocytes viability in culture for up to five days and allows for their differentiation. Using this culture system, oocytes collec
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We describe the procedures for isolation of growing oocytes from ovarian follicles at early stages of development, as well as the setup of an in vitro culture system which can support the growth and differentiation up to the fully-grown stage.