Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
3.3K Views
•
15:01 min
•
September 18th, 2021
DOI :
September 18th, 2021
•Ivan V. Maslov1, Andrey O. Bogorodskiy1, Mariia V. Pavelchenko1, Ilia O. Zykov1, Natalya I. Troyanova2, Valentin I. Borshchevskiy1,3, Marina A. Shevchenko2
1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich
Chapters in this video
0:04
Introduction
1:03
Aspergillus fumigatus Conidia Application & Specimen Preparation
1:22
Mouse Lung Lobe Optical Clearing
2:23
Mouse Lung Lobe Imaging with CLSM
6:50
Image Processing: Surface Rendering
9:23
Image Processing: Mask Correction
12:54
Conidia Quantitative Analysis
13:41
Representative Results
14:07
Conclusions
Related Videos
08:50
Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
20.6K Views
09:04
Isolation of Mouse Lung Dendritic Cells
28.9K Views
09:04
Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy
11.4K Views
12:29
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
10.1K Views
09:24
Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
7.4K Views
09:52
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
10.4K Views
09:43
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
6.7K Views
10:06
Efficient Method for Imaging Murine Lungs that Preserves Spatial Dynamics of Fungal Spores in the Airways
259 Views
11:30
Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software
3.7K Views
13:13
Confocal Fluorescence Microscopy: A Technique to Determine the Localization of Proteins in Mouse Fibroblasts
43.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved