Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

2.5K Views

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05:49 min

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April 27th, 2021

DOI :

10.3791/62478-v

April 27th, 2021

•

Devon L. Moose¹^,², Sophia Williams-Perez³, Renee Cafun¹, Benjamin L. Krog¹, Michael D. Henry¹^,²^,⁴^,⁵^,⁶

¹Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, ²Holden Comprehensive Cancer Center, University of Iowa, ³MD program, Carver College of Medicine, University of Iowa, ⁴Department of Pathology, Carver College of Medicine, University of Iowa, ⁵Department of Urology, Carver College of Medicine, University of Iowa, ⁶Department of Radiation Oncology, Carver College of Medicine, University of Iowa

Summary

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Hemodynamic Stress

Circulating Tumor Cells

Static Control Samples

Syringe Pump

Viability Assays

Enzymatic Assays

Chapters in this video

0:05

Introduction

0:39

Cell Preparation

1:41

Fluid Shear Stress (FSS) Exposure

2:49

Viability Measurement

3:59

Results: Fluid Shear Stress Resistance of Mammary Epithelial Cancer Cells

4:47

Conclusion

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