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Surgery and Sample Processing for Correlative Imaging of the Murine Pulmonary Valve

DOI :

10.3791/62581-v

August 5th, 2021

August 5th, 2021

2,364 Views

1Center for Electron Microscopy and Analysis, The Department of Materials Science and Engineering, Ohio State University, 2Center for Regenerative Medicine, Nationwide Children’s Hospital, 3Thermo Fisher Scientific

Here, we describe a correlative workflow for the excision, pressurization, fixation, and imaging of the murine pulmonary valve to determine the gross conformation and local extracellular matrix structures.

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