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Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

3.7K Views

14:34 min

December 25th, 2021

DOI :

10.3791/62791-v

December 25th, 2021


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MADS box Transcription Factor

Chapters in this video

0:08

Introduction

1:17

Cloning of Genes in Entry and Destination Vectors

2:01

Growth Conditions for Nicotiana benthamiana Plants

3:05

Prepare Bacterial Strains for Agroinfiltration

6:20

Prepare Slides for Fluorescence Visualization

6:45

Fret-Flim Analysis Using a Confocal Laser Scanning Microscope

10:51

Fret-Flim Analysis Using a Confocal Laser Scanning Microscope: The Tripartite Interaction

12:31

Representative Results

13:42

Conclusion

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