Process Development for the Production and Purification of Adeno-Associated Virus (AAV)2 Vector using Baculovirus-Insect Cell Culture System
9.1K Views
•
10:31 min
•
January 13th, 2022
DOI :
January 13th, 2022
•Chapters in this video
0:05
Introduction
0:44
Generation of Baculovirus-infected TIPS Cells
3:05
Production of AAV Vector
5:08
Lysis of Cells and Release of AAV
6:07
Purification of AAV Vector Using Affinity Column Chromatography System
8:03
Concentration and Diafiltration of AAV Vector Using Tangential Flow Filtration (TFF)
8:54
Results: Purification of Adeno-Associated Virus (AAV)2 Vector Using Baculovirus-Insect Cell Culture System
9:48
Conclusion
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