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Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms

DOI :

10.3791/63773-v

7:28 min

June 8th, 2022

June 8th, 2022

1,964 Views

1Department of Microbiology, The Ohio State University, 2Department of Microbial Infection and Immunity, The Ohio State University

The present protocol describes the study of neutrophil-biofilm interactions. Staphylococcus aureus biofilms are established in vitro and incubated with peripheral blood-derived human neutrophils. The oxidative burst response from neutrophils is quantified, and the neutrophil localization within the biofilm is determined by microscopy.

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In Vitro Assays

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