The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

2.7K Views

•

10:34 min

•

January 7th, 2022

DOI :

10.3791/63103-v

January 7th, 2022

•

Joseph J. Sherba¹, Maria Atzampou¹, Hao Lin², Jerry W. Shan², David I. Shreiber¹, Jeffrey D. Zahn¹

¹The Department of Biomedical Engineering, Rutgers, The State University of New Jersey, ²The Department of Mechanical and Aerospace Engineering, Rutgers, The State University of New Jersey

Summary

Explore More Videos

Micro electroporation

Continuous Flow System

Electroporation Pulsing

Cell Permeabilization

Chapters in this video

0:04

Introduction

0:43

Device Fabrication‐Photolithography

3:12

Device Fabrication

5:21

Cell Preparation, Experimental Setup, Operation, and Analysis

8:24

Results: Cell Membrane Permeabilization and Electro‐Transfection Efficiency Using the Continuous Flow, Micro‐Electroporation System

9:36

Conclusion

Related Videos

13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

33.2K Views

12:43

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress

43.2K Views

09:30

Bacterial Detection & Identification Using Electrochemical Sensors

28.1K Views

12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

12.2K Views

12:54

Operation of a Benchtop Bioreactor

88.7K Views

07:38

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

8.4K Views

08:29

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

8.7K Views

12:31

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry

14.9K Views

09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

7.9K Views

13:42

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

11.6K Views