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Optical Clearing of Plant Tissues for Fluorescence Imaging

DOI :

10.3791/63428-v

4:55 min

January 5th, 2022

January 5th, 2022

3,559 Views

1Institute of Transformative Bio-Molecules (ITbM), Nagoya University, 2Institute for Advanced Research (IAR), Nagoya University, 3Division of Biological Science, Graduate School of Science, Nagoya University, 4Department of Biological Sciences, Graduate School of Science, The University of Tokyo

Here, a method is described for making plant tissues transparent while maintaining the stability of fluorescent proteins. This technique facilitates deep imaging of cleared plant tissues without physical sectioning.

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Optical Clearing

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