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Deep Fluorescence Observation in Rice Shoots via Clearing Technology

DOI :

10.3791/64116-v

7:21 min

June 27th, 2022

June 27th, 2022

2,106 Views

1Graduate School of Bioagricultural Sciences, Nagoya University, 2Bioscience and Biotechnology Center, Nagoya University, 3Institute for Advanced Research (IAR), Nagoya University, 4Institute of Transformative Bio-Molecules (ITbM), Nagoya University

The present protocol describes a clearing technique for rice shoots, which are difficult to prepare for internal structural observations owing to the hard, thick, or layered nature of the tissues. This method facilitates continuous and deep fluorescence observations, even in adult rice plants.

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Deep Fluorescence Observation

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