JoVE Logo

Sign In

Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

3.0K Views

10:57 min

February 1st, 2022

DOI :

10.3791/63484-v

February 1st, 2022


Explore More Videos

Stimulated Raman Scattering

Chapters in this video

0:04

Introduction

0:49

Beam Collimation

1:32

Spatial Overlap

2:34

Electrooptical Modulation (EOM)

3:35

Temporal Overlap

5:40

Signal to Noise (SNR) Characterization, Frequency Axis Calibration, and Calibration of Spectral Resolution

7:23

EOM1 Modulation and Epi-Mode SRS Imaging

8:53

Results: Optimizing Spectral Resolution, Two-Color SRS, and False Two-Color H&E Staining of SRS Images

10:14

Conclusion

Related Videos

article

17:16

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

10.3K Views

article

09:04

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles

11.3K Views

article

09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

16.6K Views

article

11:14

Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging

12.4K Views

article

16:45

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

11.4K Views

article

09:06

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy

3.1K Views

article

07:18

Highly-Multiplexed Tissue Imaging with Raman Dyes

2.8K Views

article

08:17

Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice

7.6K Views

article

09:03

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

4.1K Views

article

04:08

Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

2.5K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved