Quantitative Analysis of Viscoelastic Properties of Red Blood Cells Using Optical Tweezers and Defocusing Microscopy
1.3K Views
•
08:03 min
•
March 25th, 2022
DOI :
March 25th, 2022
•Lucas Barreto*1,2, Fran Gomez*1,2, Pedro S. Lourenço1,2, Douglas G. Freitas1,2, Juliana Soares1,3, Clemilson Berto-Junior4,5, Ubirajara Agero6, Nathan B. Viana1,2, Bruno Pontes1,2,3,7
1Centro Nacional de Biologia Estrutural e Bioimagem - CENABIO, Universidade Federal do Rio de Janeiro, 2Instituto de Física, Programa de Pós-graduação Multidisciplinar em Física Aplicada, Universidade Federal do Rio de Janeiro, 3Instituto de Biofísica Carlos Chagas Filho, Programa de Pós-graduação em Ciências Biológicas Biofísica, Universidade Federal do Rio de Janeiro, 4Faculdade de Farmácia, Universidade Federal do Rio de Janeiro - Campus Macaé, 5Faculdade de Medicina, Programa de Pós-Graduação em Endocrinologia, Universidade Federal do Rio de Janeiro, 6Instituto de Ciências Exatas, Departamento de Física, Universidade Federal de Minas Gerais, 7Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro
Chapters in this video
0:04
Introduction
0:43
Preparation of Sample Holders and Cell Cultures
1:30
OT‐Based Rheology Experiment and Analysis
3:12
Video and Contrast Image Acquisition
5:40
Soft Glassy Rheology Model and Experimental Analysis
7:03
Results: Characterizing RBC Surface from Values of K' (ω), K'' (ω), G', and G''
7:30
Conclusion
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