A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

2.1K Views

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11:34 min

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May 10th, 2022

DOI :

10.3791/63761-v

May 10th, 2022

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Khanit Sa-ngiamsuntorn¹, Piyanoot Thongsri², Yongyut Pewkliang², Suparerk Borwornpinyo³^,⁴, Adisak Wongkajornsilp⁵

¹Department of Biochemistry, Faculty of Pharmacy, Mahidol University, ²Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, ³Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, ⁴Department of Biotechnology, Faculty of Science, Mahidol University, ⁵Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University

Summary

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Hepatocyte Model

Hepatitis B Virus

NTCP

Sodium Taurocholate Cotransporting Polypeptide

Anti HBV Compound

Viral Entry Inhibition

Flow Cytometric Analysis

Compensation Controls

Alexa Fluor 488

Cell Permeabilization

Infection Interruption

Chapters in this video

0:05

Introduction

0:50

Measuring the Sodium Taurocholate Co-Transporting Polypeptide (NTCP) Using Flow Cytometry

4:30

Anti-HBV (Hepatitis B Virus) Entry Assay

6:13

HBV (Hepatitis B Virus) -Cell Binding Assay

6:50

Taurocholic Acid Uptake Assay

7:34

Determination of Protein-Ligand Interactions Using Isothermal Titration Calorimetry

9:14

Results: Screening of Anti-Hepatitis B Virus Using Isothermal Titration Calorimetry

10:36

Conclusion

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