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Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

DOI :

10.3791/63779-v

8:14 min

December 9th, 2022

December 9th, 2022

2,716 Views

1Hyphenated Mass Spectrometry Laboratory (HyMaS), University of Technology Sydney, 2School of Life Sciences, University of Technology Sydney

The present protocol describes the extraction of non-protein amino acids from biological matrices via trichloroacetic acid (TCA) protein precipitation and acid hydrolysis before analysis using liquid chromatography-tandem mass spectrometry.

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