JoVE Logo
Faculty Resource Center

Sign In

Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis

DOI :

10.3791/63779-v

8:14 min

December 9th, 2022

December 9th, 2022

2,810 Views

1Hyphenated Mass Spectrometry Laboratory (HyMaS), University of Technology Sydney, 2School of Life Sciences, University of Technology Sydney

The present protocol describes the extraction of non-protein amino acids from biological matrices via trichloroacetic acid (TCA) protein precipitation and acid hydrolysis before analysis using liquid chromatography-tandem mass spectrometry.

Tags

Extraction

-- Views

Related Videos

article

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

article

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

article

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

article

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry

article

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

article

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

article

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

article

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

article

3D Depth Profile Reconstruction of Segregated Impurities Using Secondary Ion Mass Spectrometry

article

An Improved Technique for Trimethylamine Detection in Animal-Derived Medicine by Headspace Gas Chromatography-Tandem Quadrupole Mass Spectrometry

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved