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JoVE Journal

Biochemistry

This content is Free Access.

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

1.8K Views

11:50 min

June 23rd, 2022

June 23rd, 2022

1,773 Views

0:05

Introduction

0:47

Purification of Septin Hetero-Oligomers

4:29

Quality Control of Purity and Integrity of the Septin Hetero-Oligomer

7:34

Septin Functional Quality Control via Polymerization Analysis

8:57

Results: Analysis of Purified Septin Quality

11:01

Conclusion

Transcrição

In vitro reconstitution of cytoskeletal proteins is a vital tool to understand the basic functional properties of these proteins. The present paper describes a protocol to purify and assess the quality of recombinant septin complexes, which play a central role in cell division and migration.

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