Results: Quantification of Nuclear Markers Within Mouse Brain After Imaging on a Light-Sheet Microscope Using NuMorph
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Conclusion
Transcript
With around 100 million cells in the mouse brain and sizes of whole-brain cellular resolution images approaching the terabyte scale, advanced image analysis tools are needed to accurately quantify cells. Our computational pipeline can preprocess i
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This protocol describes methods for conducting magnetic resonance imaging, clearing, and immunolabeling of intact mouse brains using iDISCO+, followed by a detailed description of imaging using light-sheet microscopy, and downstream analyses using NuMorph.