Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
2.3K Views
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08:44 min
•
September 7th, 2022
DOI :
September 7th, 2022
•Linlin Zhang1,2, Mengjia Liu1,2, Meng Zhang1,2, Junhong Ai1,2, Jiao Tian1,2, Ran Wang1,2, Zhengde Xie1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences
Chapters in this video
0:05
Introduction
0:36
Isolation of CD14+ Monocytes from PBMCs Using CD14 Microbeads
2:38
Fluorescently Labeled Antibody Staining
3:52
Flow Cytometry Parameter Setting
4:32
Gating
6:18
Cell Sorting and Collection via Flow Cytometry
7:00
Results: Comparison of Immune Cell Subpopulations from Patients with Infectious Mononucleosis, Healthy Epstein Barr Virus Carriers, and Uninfected Samples
8:14
Conclusion
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