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Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester

DOI :

10.3791/52983-v

July 7th, 2015

July 7th, 2015

8,528 Views

1Department of Neurology, University of Washington, 2Therapeutic Innovation Group, School of Life and Medical Sciences, University College London, 3Department of Neuroinflammation, University College London

Microglia can influence neurons and other glia in culture by various non-cell autonomous mechanisms. Here, we present a protocol to selectively deplete microglia from primary neuronal cultures. This method has the potential to elucidate the role of microglial-neuronal interactions, with implications for neurodegenerative conditions where neuroinflammation is a hallmark feature.

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Microglia

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