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Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

DOI :

10.3791/64756-v

June 9th, 2023

June 9th, 2023

971 Views

1Department of Molecular Pathology, The Netherlands Cancer Institute, 2Oncode Institute, 3Animal Laboratory Facility, The Netherlands Cancer Institute

Here, we present a method to obtain images of the intestine upon laser-induced wounding. By exposing the mouse intestine to a multiphoton laser, the loss of a single or multiple crypt(s) is induced locally. By repeatedly imaging the damaged area over months, the real-time dynamics of intestinal recovery is captured.

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Laser Ablation

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