Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

2.5K Views

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07:14 min

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June 16th, 2023

DOI :

10.3791/64775-v

June 16th, 2023

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Pavan S. Krishnan¹^,², Fernando T. Zamuner¹^,³, Carolyn M. Jenks¹, Johnny Y. Xie⁴, Lisa Zhang⁵, Mohammed Lehar¹, Neal S. Fedarko⁶, Mariana Brait¹^,³, John P. Carey¹

¹Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, ²Virginia Commonwealth University School of Medicine, ³Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, ⁴Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, ⁵Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, ⁶ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine

Summary

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CGRP

Calcitonin Gene related Peptide

Human Plasma

ELISA Protocol

Quantification

Solid Phase Extraction

Protease Degradation

Blocking Buffer

Biomarker

Migraine Pathophysiology

Chapters in this video

0:04

Introduction

1:03

Collection, Preparation, and Extraction of Plasma Sample

3:28

ELISA Plate Preparation (Blocking)

4:16

Enzyme-Linked Immunosorbent Assay (ELISA)

6:06

Results: Effect of Blocking on CGRP Quantification

6:29

Conclusion

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