Collection, Preparation, and Extraction of Plasma Sample
3:28
ELISA Plate Preparation (Blocking)
4:16
Enzyme-Linked Immunosorbent Assay (ELISA)
6:06
Results: Effect of Blocking on CGRP Quantification
6:29
Conclusion
Transcript
This modified ELISA protocol allows for the purification and accurate quantification of CGRP in human plasma. It has undergone necessary validation experiments and can serve to standardize future quantification methodologies. This protocol offers
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Published data pertaining to calcitonin gene-related peptide (CGRP) concentrations in human plasma are inconsistent. These inconsistencies may be due to the lack of a standardized, validated methodology to quantify this neuropeptide. Here, we describe a validated enzyme-linked immunosorbent assay (ELISA) protocol to purify and quantify CGRP in human plasma.