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Isolating and Culturing Vestibular and Spiral Ganglion Somata from Neonatal Rodents for Patch-Clamp Recordings

1.0K Views

11:05 min

April 21st, 2023

DOI :

10.3791/64908-v

April 21st, 2023


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Vestibular Ganglion

Chapters in this video

0:00

Introduction

1:09

Fabricating Trituration Pipettes

1:52

Extracting the Superior Vestibular Ganglion

4:01

Tissue Dissociation and Plating

5:24

Extracting the Spiral Ganglion

6:58

Patch-Clamp Recordings

8:31

Results: Voltage-Clamp Protocols to Measure Diverse Groups of Ionic Currents and Diverse Firing Patterns

10:25

Conclusion

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