Reusable Single Cell for Iterative Epigenomic Analyses

1.2K Views

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10:28 min

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February 11th, 2022

DOI :

10.3791/63456-v

February 11th, 2022

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Hidetaka Ohnuki¹, David J. Venzon², Alexei Lobanov³^,⁴, Giovanna Tosato¹

¹Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, ²Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, ³CCR Collaborative Bioinformatics Resource (CCBR), Center for Cancer Research, National Cancer Institute, National Institutes of Health, ⁴Advanced Biomedical Computational Science, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute

Summary

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Transcription Factors

Single cell Epigenome

Epigenetic Therapy

Fluorescence Microscopy

Liquid Handling Robot

Chapters in this video

0:05

Introduction

1:32

Preparation of Reusable Single Cells: Manual Version

3:09

Random Primer Annealing and Extension

4:09

Antibody Binding and Proximity Ligation of Antibody Probe and Proximally Extended Random Primer

5:25

Full Extension of the 1^st Random Primer and Multiple Displacement Amplification

7:32

Results: Single Cell Multiepigenomic Analysis

9:26

Conclusion

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