使用 CRISPR/Cas9 系统生成着丝粒相关蛋白- E ^CENP-E-/- 敲除细胞系

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11:49 min

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June 23rd, 2023

DOI :

10.3791/65476-v

June 23rd, 2023

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Meng-Fei Xu¹^,², Jie Chen¹^,², Yue Xu¹^,², Jing-Lian Zhang¹^,², Yi Zhou¹^,², Jie-Jie He¹^,², Shan Wu¹^,², Ya-Lan Wei³^,⁴, Zhen-Yu She¹^,²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, ⁴Medical Research Center, Fujian Maternity and Child Health Hospital

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196 CENP E E HeLa BUB1 B BubR1

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0:00

Introduction

2:16

Transfection, Isolation, and Screening of the CENP‐E Knockout HeLa Cells

6:25

Immunofluorescence Staining and High‐Resolution Confocal Microscopy of CRISPR/Cas9‐Mediated CENP‐E Knockout HeLa Cells