Preparation of the Multi‐Well Device for Media Loading
3:29
Filling the Wells with Low Melting Nematode Growth Media (lmNGM)
5:53
Adding Copper Sulfate to the Moat and Adding Autoclaved Water Crystals
6:55
Spotting the Wells with Concentrated Bacteria
7:29
Adding Worms to the Multi‐Well Device and Finishing the Preparation of the Device for Long‐Term Use
8:00
Results: Correlation of Measured Phenotypes Across Populations in Individual Animals
8:55
Conclusion
Transcript
When we're studying aging and sea elegans we're typically following a population of animals over time. This gives us a glimpse of what that process you're studying is doing in the population at each time point but it doesn't tell you how the proce
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Presented here is an optimized protocol for culturing isolated individual nematodes on solid media in microfabricated multi-well devices. This approach allows individual animals to be monitored throughout their lives for a variety of phenotypes related to aging and health, including activity, body size and shape, movement geometry, and survival.