The aim of our research is to develop an artificial microenvironment system for coral culture and study. The microenvironment in the micro-device system can at least be semi-quantitatively controlled. At the present, the existing equipment and system used for coral culture lacks precise control over temperature and other environmental factors.
Additionally, these systems are open, making them susceptible to contamination from airborne substance. Our indoor coral culture system boasts superior control accuracy and microbial tightness compared to other similar systems or tanks. Additionally, our system is commercially cost-effective.
Using this micro-device system, we can somehow customize the environmental parameters like temperature, ionic strength or metabolic concentration, which will make the coral study more quantitative and might promote the current coral research. We would like to know about the longevity of corals in this artificial micro-device. Another important question that we want to address is how the artificially built-up system affects the growth process of corals in comparison to their natural environment.
To begin, connect all the modules and pumps. Ensure the water flows circularly over the system for a minimum of 30 minutes. Inspect all the seams for potential leaks.
After conducting the tightness test, evacuate and dry the water inside. Then load the appropriate contents in the modules. Fix the modules onto the baseboard using the screws, and connect the inner circulation modules to the outer circulation modules.
Then inject the seawater into the water inlet of the seawater store module. Once the water level reaches three centimeters above the water inlets of the pumps, switch them on and continue injecting the seawater. Now turn on all the switches and set the seawater pump voltages to nine volts.
Set the water temperature to 25 degrees Celsius. Next, set the cycle timer to one minute on and one minute off. Set all three channels of the three-channel timer to 9:00 AM on and 5:00 PM off.
After setting the micro-drive system, add one milliliter of the commercial microbiome source solution into 500 milliliters of the seawater while stirring. Next, inject 50 milliliters of the diluted solution and 10 microliters of the commercial coral nutrition solution into the circulation system. Switch on the inner circulation pump and turn on the air pump to culture the microbiome for 21 days.
Cut the rock coral branches into a length of three to five centimeters. Adhere these coral branches onto 3D-printed coral support bases. Place the coral branch samples back into the original seawater tank for a minimum of seven days for recovery.
Fix the coral support base onto the rotation unit using glue. Assemble the coral culture module and connect it to the outer circulation loop. Position the camera on the top of the studio and capture the images from a vertical view.
Next, place the coral culture module in the mini-photo studio with the coral positioned in the center and at the bottom. Capture coral images by rotating the outside handle. The coral culture module effectively controlled the seawater temperature and prevented temperature fluctuation, which can strongly affect coral survival.
The sample corals'tentacles extended over one month, indicating that the system provided a suitable survival environment for the coral. To begin, perform leakage inspection for system safety by inspecting the baseboard, seam, and connectors for water stains or droplets. The transparent nature of the system's cover shell facilitates visual inspection of water leakage.
Extract 10 milliliters of seawater using a syringe from the three-way valve between the activated charcoal and seawater module. Dissolve the additives in the extracted seawater and inject the solution back into the system through the three-way valve to replenish the system with nutrients and other reagents. To reduce toxic concentration and eutrophication in the culture system, exchange water by switching off the power of the entire system and unplugging the power cable for safety.
Then remove the coral culture module and connect the outside wastewater pipeline to the outlet in the seawater store module. Now rotate the system and position it front-side down. Turn on the outlet and allow the seawater to flow out from inside the system.
Discharge the required amount of seawater and take off the outlet. Afterwards, reset the system and inject the newly-prepared seawater through the water inlet. Re-install the coral culture module into the system.
Switch on the system and wait for the system to get back to normal.
