JoVE Logo
Faculty Resource Center

Sign In

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

DOI :

10.3791/50971-v

7:33 min

January 3rd, 2014

January 3rd, 2014

19,584 Views

1Wageningen UR Plant Breeding, Wageningen University, 2Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Ministry of Education, National Centre for Vegetable Improvement (Central China), Potato Engineering and Technology Research Centre of Hubei Province, Huazhong Agricultural University

Agroinfiltration and PVX agroinfection are routine functional assays for transient ectopic expression of genes in plants. These methods are efficient assays in effectoromics strategies (rapid resistance and avirulence gene discovery) and crucial to modern research in molecular plant pathology. They meet the demand for robust high-throughput functional analysis in plants.

Tags

Keywords Agroinfiltration

-- Views

Related Videos

article

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

article

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

article

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

article

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

article

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

article

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

article

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana

article

A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response (Video) | JoVE

article

An Experimental Study on Colorado Potato Beetle Hibernation Under Natural Conditions

article

Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved