In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
September 2nd, 2008
•Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.
Related Videos
Preparation and Fractionation of Xenopus laevis Egg Extracts
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
Facial Transplants in Xenopus laevis Embryos
Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation
Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
A Cell-Free Assay Using Xenopus laevis Embryo Extracts to Study Mechanisms of Nuclear Size Regulation
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved