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In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts (Video) | JoVE

DOI :

10.3791/908-v

4:49 min

September 2nd, 2008

September 2nd, 2008

11,042 Views

1Department of Cell Biology, Emory University

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

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In Vitro Nuclear Assembly

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