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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

DOI :

10.3791/50434-v

10:01 min

June 27th, 2013

June 27th, 2013

13,328 Views

1Department of Molecular Biology, Massachusetts General Hospital, 2Department of Genetics, Harvard Medical School

We describe the collection of unfertilized Xenopus tropicalis eggs and production of a meiosis II-arrested egg extract. This egg extract can be used to purify microtubules and microtubule-associated RNAs.

Tags

Xenopus Tropicalis

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