We thank Laurie Lee for critical reading of the manuscript. T.W.C is supported by an American Heart Association Predoctoral Fellowship (12PRE6590007). M.R.B. is supported by a National Cancer Institute training grant (T32 CA119925). S.S.H is supported by National Institutes of Health (R01DK078640). E.L. is supported by the National Institutes of Health (R01GM081635 and R01GM103926).

1. Preparation of Xenopus&#160;Egg Extract
NOTE: Each frog yields approximately 1 ml&#160;of usable egg extract. Extracts from 10 frogs are typically prepared at one time, and the volume of buffer described below is for performing a 10 frog Xenopus egg extract prep. The buffer volume can be adjusted accordingly for larger or smaller preparations of egg extract. Preps generated in this manner consistently yield protein concentrations &#8805; 50 mg/ml. The process of collecting eggs and processing them into extract is most efficient when conducted by two people. (For basic frog husbandry techniques, see Sive&#160;et al.33).
<ol>
	<li>Egg Collection
	<ol>
		<li>To prime the frogs, inject each female frog with 100 U of Pregnant Mare Serum Gonadotropin (PMSG) from a freshly made 250 U/ml&#160;stock. Use a 3 ml&#160;tuberculin syringe with a 27 G&#160;needle to inject subcutaneously, with the bevel of the needle up, into the dorsal lymph sac, which is located approximately 1 cm away from the midline from the notched discolorations along the length of the legs of the frog.</li>
		<li>Store primed frogs in water (plus 20 mM NaCl, see&#160;Sive&#160;et al.33) at 18 &#176;C for 5-10 days. For standing water tank systems, the animal density is approximately 4 L of water per female frog. NOTE: The minimum time required for priming to take effect is 5 days, and the effects of priming wear off after 10 days.</li>
		<li>Prepare 0.5x Marc&#8217;s Modified Ringers (MMR) solution from a 20x MMR stock. 20x MMR consists of 2 M sodium chloride, 40 mM potassium chloride, 40 mM calcium chloride, 20 mM magnesium chloride, and 100 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), pH 7.4.</li>
		<li>Set up buckets for all injected frogs (one frog per 4 L bucket). NOTE: Although more than one frog can be placed in the same bucket for egg collection, if one of the frogs lays predominantly poor quality eggs, a substantial amount of effort will be required to separate the poor quality eggs from those that are suitable for making extract. Thus, maximizing the number of frogs in the same tank to minimize the amount of buffer used for egg collect is not worth the risk.</li>
		<li>Inject 750 U Human Chorionic Gonadotropin (HCG) into the dorsal lymph sac of each frog using a 27 G needle as described in 1.1.1.</li>
		<li>Place each of the HCG-injected frogs into individual 4 L buckets containing 0.5x MMR cooled to 16 &#176;C.</li>
		<li>Place the containers with the frogs in a 16 &#176;C incubator to collect eggs O/N (15-16 hr). NOTE: Maintaining the proper temperature is critical for the entire procedure, from collecting eggs to preparing egg extract.</li>
	</ol>
	</li>
	<li>Dejellying Eggs 
	NOTE: Eggs are covered with a jelly coat that must be removed prior to making extract. The likelihood of spontaneous lysis of the eggs increases as the time between egg laying and extract preparation increases. Thus, it is important to proceed through the following steps as rapidly as possible.
	<ol>
		<li>Prepare 4 L of 1x MMR, 50 ml&#160;of 0.1x MMR, and 400 ml&#160;of 2% cysteine, pH 7.7, made in distilled water. Maintain all solutions at 16 &#176;C.</li>
		<li>To expel additional eggs, gently squeeze the lower back and abdomen of the frog.</li>
		<li>Remove the frogs and the bulk of the MMR, to leave the eggs in approximately 1-200 ml of MMR in each bucket.</li>
		<li>Remove debris with a transfer pipette, and assess the quality of the eggs: high quality eggs are generally marked by a clear separation between the darkly pigmented animal hemisphere and the lightly colored vegetal hemisphere and have the highest dark-to-light contrast. Discard with a transfer pipette any eggs that appear stringy, mottled, or lysed (white and puffy) as they will decrease the overall quality of the extract. If &#62;10% of the eggs are of poor quality, the entire batch should be discarded.</li>
		<li>Combine eggs into a 500 ml glass beaker and pour out as much MMR as possible while keeping eggs submerged.</li>
		<li>Rinse eggs by gentle swirling with twice the egg volume of MMR. Repeat twice, and remove any debris or obviously poor quality eggs.</li>
		<li>Add approximately 100 ml of 2% cysteine to the glass beaker, swirl gently to mix, and allow eggs to settle for 5 min&#160;at 16 &#176;C. Pour off the cysteine. NOTE: Dejellying is marked by the gradual appearance of jelly coats floating above the eggs and the more compact packing of the eggs as they now occupy a smaller volume without the jelly coat.</li>
		<li>Add another 100 ml of 2% cysteine, gently swirl, wait 5 min, and then slowly pour off the cysteine. Repeat until eggs have become tightly compacted (usually by the third cysteine treatment). Note: If eggs are left too long in cysteine, they are prone to lysis. Similarly, dejellied eggs are fragile and prone to mechanical lysis if they are swirled too vigorously or if they are exposed to air. Once the eggs have been dejellied, it is important to rapidly proceed to the centrifugation steps.</li>
		<li>Pour off cysteine, and rinse away the jelly coat and other debris by gently washing eggs in 1x MMR. Pour off buffer carefully along the side of the beaker. Repeat twice&#160;or until MMR solution is no longer cloudy. While rinsing with 1x MMR continue to remove the bad eggs using a transfer pipette.</li>
		<li>Perform a final gentle rinse with 30 ml 0.1x MMR, and gently pour off as much of the buffer as possible. Again, remove any obviously bad eggs.</li>
	</ol>
	</li>
	<li>Packing and Crushing Eggs by Centrifugation 
	NOTE: The extract described below that is used for &#946;-catenin degradation is a variant of the cytostatic factor extract (metaphase II-arrested). In contrast to the low-speed and high-speed extract used for cell cycle studies, intermediate speed extract works best for &#946;-catenin degradation. Interphase extract similarly promotes robust &#946;-catenin degradation although is more labor intensive to prepare.
	<ol>
		<li>Add Leupeptin, Pepstatin, Aprotinin mixture (LPA, a protease inhibitor) at 10 &#181;g/ml (diluted from a 10 mg/ml stock solution in DMSO) and Cytochalasin D at 20 &#181;g/ml (diluted from a 10 mg/ml stock solution in DMSO) into the remaining 20 ml of 0.1x MMR.</li>
		<li>Add 0.&#160;x MMR containing LPA and Cytochalasin D to the washed eggs, swirl gently, and incubate for 5 min&#160;at 16 &#176;C.</li>
		<li>Transfer eggs into 16 &#176;C pre-chilled 50 ml centrifuge tubes, allow the eggs to settle, and remove residual buffer from the top. To prevent exposure to air, withdraw a small amount of buffer into the transfer pipette prior to withdrawing eggs for transfer. Continue to transfer additional eggs into the centrifuge tubes and remove residual buffer from the top of the tube until the eggs fill the centrifuge tube to the top, which will maximize the yield of extract.</li>
		<li>To pack the eggs, spin centrifuge tubes at 400 x g for 60 sec&#160;at 4 &#176;C using a fixed angle rotor. Remove residual buffer from the top of the centrifuge tubes.</li>
		<li>For the crushing spin, spin tubes at 15,000 x g for 5 min&#160;at 4 &#176;C.</li>
	</ol>
	</li>
	<li>Collecting Cytoplasmic Layer of Extract 
	NOTE: The method described below differs from the classic method of puncturing the side of the centrifuge tube in order to collect the cytoplasmic layer. This protocol has been adapted for simplification and for use with particular centrifuge tubes, which are reusable. No noticeable differences in the kinetics of &#946;-catenin degradation are found with either method. At this point extract should be kept cold during throughout the process, and all steps should be performed at 4 &#176;C.
	<ol>
		<li>Clear a hole in the lipid layer using P1000 pipette&#160;tip.</li>
		<li>Collect the cytoplasmic layer (between the dark pigmented layer and the light lipid layer) using a new P1000 pipette tip&#160;into clean pre-chilled centrifuge tubes (Figure 1). For high-quality extract that robustly degrades &#946;-catenin, minimize the amount of pigmented and lipid layer that is withdrawn with the cytoplasmic layer.</li>
		<li>Spin extracted cytoplasmic layer at 15,000 x g for 10 min&#160;at 4 &#176;C and again collect the cytoplasmic layer. Repeat the spin and extraction 1X. NOTE: The extract should be &#34;straw colored.&#34; If there is substantial contamination with the pigmented and lipid layers at this point, one can repeat the spin one more time, although excessive spins will decrease the capacity of the extract to degrade &#946;-catenin.</li>
		<li>Add LPA and Cytochalasin D to the extract at final concentrations of 10 &#181;g/ml each. NOTE: Using the method described yields a fairly consistent total protein concentration of approximately 50 mg/ml (52.41 &#177; 5.40 mg/ml, n=3 separate preps) in Xenopus egg extracts.</li>
		<li>(Optional) For translation assays, add capped mRNA (0.1 mg/ml), RNAsin (1.5 U/ml), and energy regeneration mix (2.2.1) and incubate the reaction at RT for 2 hr (for further information see Salic&#160;et al.2 and Sive&#160;et al.33). Use translated extract immediately for &#946;-catenin degradation assays or snap-freeze in liquid nitrogen for later use. NOTE: Freshly prepared extract has a high capacity to translate exogenously added mRNA, but unfortunately, this capacity is lost once the extract is frozen. Capped mRNA can be readily prepared using commercially available kits.</li>
		<li>Snap-freeze extract in liquid nitrogen. NOTE: Extracts are stored in small (200 &#181;l) aliquots for single use because they rapidly lose their capacity to degrade &#946;-catenin if refrozen. For long-term storage, extract can be stored in liquid nitrogen. For short-term storage, extract can be stored at -80 &#176;C, although the capacity of extract to degrade &#946;-catenin can be dramatically reduced with extended storage at -80 &#176;C (longer than 2 months).</li>
	</ol>
	</li>
</ol>
2. Preparing Extract for &#946;-catenin Degradation Assay
<ol>
	<li>Depletion from Xenopus&#160;Extract 
	NOTE: A major advantage of Xenopus extract is the capacity to readily deplete components of a pathway and precisely add back a defined amount of a protein in order to determine its dose-dependent effects.
	<ol>
		<li>Use freshly prepared Xenopus egg extract or quickly thaw frozen extract and place on ice. Perform all manipulations in the cold.</li>
		<li>Add extract to 1/10 the volume of pelleted antibody or affinity beads (e.g., 20 ml pelleted beads to 200 ml extract). In order to minimize dilution of the extract, withdraw as much liquid from the beads as possible before addition of the extract using gel loading tips with long, tapered tips.</li>
		<li>Rotate extract-bead mix at 4 &#176;C for 1 hr.</li>
		<li>Spin extract-bead mix at 12,600 x g in microfuge at 4 &#176;C for 30 sec. Alternatively, if magnetic beads are used, apply magnetic field to collect beads.</li>
		<li>Transfer depleted extract to a fresh microfuge tube on ice. Be careful not to transfer any beads with the extract.</li>
		<li>Confirm efficiency of depletion by immunoblotting both depleted extract and beads.</li>
		<li>Prepare extract for &#946;-catenin degradation assay as described in 2.2.</li>
	</ol>
	</li>
	<li>Optimizing Xenopus&#160;Extract for &#946;-catenin Degradation 
	NOTE: &#946;-catenin degradation in Xenopus egg extract is an energy-dependent process that quickly depletes the endogenous ATP stores. Consequently, an energy regeneration system is required to maintain robust &#946;-catenin degradation.
	<ol>
		<li>Prepare a 20x energy regeneration (ER) mix consisting of 150 mM creatine phosphate, 20 mM ATP, 600 &#181;g/ml creatine phosphokinase, and 20 mM MgCl2. ER should be aliquoted and stored at -80 &#176;C. Avoid repeated freeze/thaw cycles by using small frozen aliquots.</li>
		<li>Quickly thaw Xenopus egg extract by rubbing the frozen tube between hands. Place the tube on ice just before all of the extract has melted.</li>
		<li>Add 10 &#181;l&#160;of energy regeneration mix (20x ER) into an aliquot (200 &#956;l) of Xenopus egg extract. Mix thoroughly by quickly flicking the tube and pulse vortexing. Pulse-spin and immediately place on ice.</li>
		<li>(optional) The turnover of &#946;-catenin can be slightly enhanced in Xenopus egg extract by addition of ubiquitin (1.25 mg/ml final). Cycloheximide (0.1 mg/ml final) can also be added to minimize translation of endogenous transcripts.</li>
		<li>Aliquot the appropriate volumes for degradation assay into pre-chilled microfuge tubes on ice. For radiolabeled &#946;-catenin degradation assays, withdraw 2-5 ml extract for each time point.</li>
	</ol>
	</li>
</ol>
3. Radiolabeled &#946;-catenin degradation assay in Xenopus egg extract
NOTE: All steps should be performed on ice unless otherwise indicated.
<ol>
	<li>Preparing Radiolabeled &#946;-catenin
	<ol>
		<li>Prepare freshly in vitro-synthesized [35S]methionine-radiolabeled protein using commercially available kits. NOTE: Generating 35S-labeled (half-life 87 days) proteins are easily and efficiently produced using commercially available in vitro-coupled transcription-translation kits. It is important that the translated protein is sufficiently labeled such that changes in protein turnover can be readily visualized.</li>
		<li>To confirm successful radiolabeling, perform SDS-PAGE/autoradiography with 0.5 &#181;l&#160;of the translated protein. Quantify the intensity of the radiolabeled &#946;-catenin band using ImageJ, ImageQuant, or an alternative quantitative software program. NOTE: The radiolabeled &#946;-catenin protein band should be clearly visible on film within a few hours (4-6 hr).</li>
		<li>Snap-freeze the radiolabeled protein in liquid nitrogen for storage until use. Note: Prolonged storage (&#62;2 months) and multiple freeze/thaw (greater than 2) can severely impact the capacity of the radiolabeled &#946;-catenin to degrade robustly in Xenopus egg extract. Typically, the stability of radiolabeled proteins declines significantly after 1&#160;month of storage at -80 &#176;C; thus, relatively freshly prepared radiolabeled &#946;-catenin gives best results in these degradation assays.</li>
	</ol>
	</li>
	<li>Performing &#946;-catenin Degradation Assay
	<ol>
		<li>Add 1-3 &#181;l&#160;(depending on the strength of the radiolabeled band signal) of in vitro-translated &#946;-catenin (and other proteins, small molecules, etc. that are being tested) into 20 &#181;l&#160;of Xenopus reaction mix on ice. Mix thoroughly by quickly flicking the tube and a short pulse of vortexing; this is an important step as Xenopus egg extract is very viscous, and incomplete mixing will affect the consistency of the results. Pulse spin and place on ice.</li>
		<li>Start the &#946;-catenin degradation reaction by shifting the tubes to RT.</li>
		<li>At the designated time point, remove 1-5 ml of the sample and mix immediately with SDS sample buffer (5x volume) to stop the reaction. To make sure the degradation reaction is completely terminated, flick tube several times and vortex vigorously.</li>
		<li>Perform SDS-PAGE/autoradiography. Run 1 ml&#160;equivalents (~50 mg of protein) of the extract for each time point/lane. Degradation of &#946;-catenin in Xenopus egg extract should be evidenced by the time-dependent decrease in intensity of the radiolabeled &#946;-catenin band Figure 2. Quantify results using ImageJ, ImageQuant, or other preferred imaging software if necessary.</li>
		<li>(optional) Soak SDS-polyacrylamide gel in fixing solution (10% acetic acid and 30% methanol in distilled water) prior to drying to decrease background radioactivity and increase the quality of the image.</li>
	</ol>
	</li>
</ol>
4. &#946;-catenin-luciferase Degradation Assay in Xenopus Egg Extract
Perform all steps on ice unless otherwise indicated.
<ol>
	<li>Preparing &#946;-catenin-luciferase
	<ol>
		<li>Synthesize non-radiolabeled, luciferase-tagged &#946;-catenin using the transcription-translation coupled system with complete amino acid mix.</li>
		<li>Confirm production of the luciferase-tagged &#946;-catenin by measuring luciferase activity from 0.5-1 &#181;l&#160;of the reaction. Assess background luminescence by measuring luminescence from an untranslated reaction mix. NOTE: Multiple commercial kits are available for measuring luciferase activity. Long-lived luminescence, however, works particularly well for the degradation assay.</li>
	</ol>
	</li>
	<li>Performing &#946;-catenin-luciferase Degradation Assay
	<ol>
		<li>Thaw and prepare Xenopus egg extract as in 2.2.</li>
		<li>Add in vitro-translated &#946;-catenin-luciferase fusion (from 4.1) into prepared Xenopus reaction mix (from 2.2) on ice and mix well as in 3.2.1. NOTE: The activity of the &#946;-catenin luciferase that is added to the extract is typically between 20 - 50,000 relative luminescence units (RLU)/ml of extract (based on measurements obtained from 4.1.2). Starting signal should be approximately 100,000 RLU (2-5 ml of the in vitro-translated &#946;-catenin-luciferase fusion).</li>
		<li>Shift the extract to RT to start the degradation reaction.</li>
		<li>Remove an aliquot of the reaction at the indicated time and snap-freeze in liquid nitrogen. NOTE: Triplicate samples are typically removed for analysis for each time point. Frozen extract can be stored at -80 &#176;C until they are ready to be analyzed.</li>
		<li>Thaw samples ice, transfer samples to standard white 96-well plates on ice, and process for luciferase activity.</li>
	</ol>
	</li>
</ol>

<ol>
	<li>Glotzer, M., Murray, A. W., Kirschner, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cyclin+is+degraded+by+the+ubiquitin+pathway.">Cyclin is degraded by the ubiquitin pathway.</a> Nature. 349, 132-138 (1991).</li><li>Salic, A., Lee, E., Mayer, L., Kirschner, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Control+of+beta-catenin+stability:+reconstitution+of+the+cytoplasmic+steps+of+the+wnt+pathway+in+Xenopus+egg+extracts.">Control of beta-catenin stability: reconstitution of the cytoplasmic steps of the wnt pathway in Xenopus egg extracts.</a> Molecular Cell. 5, 523-532 (2000).</li><li>Murray, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+cycle+extracts.">Cell cycle extracts.</a> Methods in cell biology. 36, 581-605 (1991).</li><li>Dabauvalle, M. C., Scheer, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assembly+of+nuclear+pore+complexes+in+Xenopus+egg+extract.">Assembly of nuclear pore complexes in Xenopus egg extract.</a> Biology of the cell / under the auspices of the European Cell Biology Organization. 72, 25-29 (1991).</li><li>Tutter, A. V., Walter, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chromosomal+DNA+replication+in+a+soluble+cell-free+system+derived+from+Xenopus+eggs.">Chromosomal DNA replication in a soluble cell-free system derived from Xenopus eggs.</a> Methods Mol Biol. 322, 121-137 (2006).</li><li>Theriot, J. A., Rosenblatt, J., Portnoy, D. A., Goldschmidt-Clermont, P. J., Mitchison, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Involvement+of+profilin+in+the+actin-based+motility+of+L.+monocytogenes+in+cells+and+in+cell-free+extracts.">Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts.</a> Cell. 76, 505-517 (1994).</li><li>Maresca, T. J., Heald, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methods+for+studying+spindle+assembly+and+chromosome+condensation+in+Xenopus+egg+extracts.">Methods for studying spindle assembly and chromosome condensation in Xenopus egg extracts.</a> Methods Mol Biol. 322, 459-474 (2006).</li><li>Shennan, K. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Xenopus+egg+extracts:+a+model+system+to+study+proprotein+convertases.">Xenopus egg extracts: a model system to study proprotein convertases.</a> Methods Mol Biol. 322, 199-212 (2006).</li><li>Kornbluth, S., Yang, J., Powers, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+the+cell+cycle+using+Xenopus+egg+extracts.">Analysis of the cell cycle using Xenopus egg extracts.</a> Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]. 11, (2006).</li><li>Chan, R. C., Forbes, D. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+study+of+nuclear+assembly+and+nuclear+import+using+Xenopus+egg+extracts.">In vitro study of nuclear assembly and nuclear import using Xenopus egg extracts.</a> Methods Mol Biol. 322, 289-300 (2006).</li><li>Masui, Y., Markert, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytoplasmic+control+of+nuclear+behavior+during+meiotic+maturation+of+frog+oocytes.">Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.</a> The Journal of experimental zoology. 177, 129-145 (1971).</li><li>Forbes, D. J., Kirschner, M. W., Newport, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spontaneous+formation+of+nucleus-like+structures+around+bacteriophage+DNA+microinjected+into+Xenopus+eggs.">Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.</a> Cell. 34, 13-23 (1983).</li><li>Lohka, M. J., Masui, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Formation+in+vitro+of+sperm+pronuclei+and+mitotic+chromosomes+induced+by+amphibian+ooplasmic+components.">Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.</a> Science. 220, 719-721 (1983).</li><li>Newport, J. W., Kirschner, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+the+cell+cycle+during+early+Xenopus+development.">Regulation of the cell cycle during early Xenopus development.</a> Cell. 37, 731-742 (1984).</li><li>Mitchison, T., Kirschner, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamic+instability+of+microtubule+growth.">Dynamic instability of microtubule growth.</a> Nature. 312, 237-242 (1984).</li><li>Blow, J. J., Laskey, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Initiation+of+DNA+replication+in+nuclei+and+purified+DNA+by+a+cell-free+extract+of+Xenopus+eggs.">Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.</a> Cell. 47, 577-587 (1986).</li><li>Verma, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ubistatins+inhibit+proteasome-dependent+degradation+by+binding+the+ubiquitin+chain.">Ubistatins inhibit proteasome-dependent degradation by binding the ubiquitin chain.</a> Science. 306, 117-120 (2004).</li><li>Yu, H., King, R. W., Peters, J. M., Kirschner, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+a+novel+ubiquitin-conjugating+enzyme+involved+in+mitotic+cyclin+degradation.">Identification of a novel ubiquitin-conjugating enzyme involved in mitotic cyclin degradation.</a> Curr Biol. 6, 455-466 (1996).</li><li>Thorne, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small-molecule+inhibition+of+Wnt+signaling+through+activation+of+casein+kinase+1alpha.">Small-molecule inhibition of Wnt signaling through activation of casein kinase 1alpha.</a> Nature Chemical Biology. 6, 829-836 (2010).</li><li>Thorne, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+biochemical+screen+for+identification+of+small-molecule+regulators+of+the+Wnt+pathway+using+Xenopus+egg+extracts.">A biochemical screen for identification of small-molecule regulators of the Wnt pathway using Xenopus egg extracts.</a> Journal of Biomolecular Screening. 16, 995-1006 (2011).</li><li>Hinkson, I. V., Elias, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+dynamic+state+of+protein+turnover:+It's+about+time.">The dynamic state of protein turnover: It's about time.</a> Trends in Cell Biology. 21, 293-303 (2011).</li><li>Kinzler, K. W., Vogelstein, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lessons+from+Hereditary+Colorectal+Cancer.">Lessons from Hereditary Colorectal Cancer.</a> Cell. 87, 159-170 (1996).</li><li>Lee, E., Salic, A., Kirschner, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physiological+regulation+of+[beta]-catenin+stability+by+Tcf3+and+CK1epsilon.">Physiological regulation of [beta]-catenin stability by Tcf3 and CK1epsilon.</a> J Cell Biol. 154, 983-993 (2001).</li><li>Lee, E., Salic, A., Kr&#252;ger, R., Heinrich, R., Kirschner, M. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+roles+of+APC+and+Axin+derived+from+experimental+and+theoretical+analysis+of+the+Wnt+pathway.">The roles of APC and Axin derived from experimental and theoretical analysis of the Wnt pathway.</a> PLoS Biology. 1, (2003).</li><li>Seeling, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+beta-catenin+signaling+by+the+B56+subunit+of+protein+phosphatase+2A.">Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A.</a> Science. 283, 2089-2091 (1999).</li><li>Guger, K. A., Gumbiner, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=beta-Catenin+has+Wnt-like+activity+and+mimics+the+Nieuwkoop+signaling+center+in+Xenopus+dorsal-ventral+patterning.">beta-Catenin has Wnt-like activity and mimics the Nieuwkoop signaling center in Xenopus dorsal-ventral patterning.</a> Dev Biol. 172, 115-125 (1995).</li><li>Cselenyi, C. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=LRP6+transduces+a+canonical+Wnt+signal+independently+of+Axin+degradation+by+inhibiting+GSK3's+phosphorylation+of+&#946;-catenin.">LRP6 transduces a canonical Wnt signal independently of Axin degradation by inhibiting GSK3's phosphorylation of &#946;-catenin.</a> Proceedings of the National Academy of Sciences. 105, 8032-8037 (2008).</li><li>Jernigan, K. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gbetagamma+activates+GSK3+to+promote+LRP6-mediated+beta-catenin+transcriptional+activity.">Gbetagamma activates GSK3 to promote LRP6-mediated beta-catenin transcriptional activity.</a> Science Signaling. 3, (2010).</li><li>Major, M. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wilms+tumor+suppressor+WTX+negatively+regulates+WNT/beta-catenin+signaling.">Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling.</a> Science. 316, 1043-1046 (2007).</li><li>Cross, M. K., Powers, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Obtaining+eggs+from+Xenopus+laevis+females.">Obtaining eggs from Xenopus laevis females.</a> J Vis Exp. , (2008).</li><li>Cross, M. K., Powers, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preparation+and+fractionation+of+Xenopus+laevis+egg+extracts.">Preparation and fractionation of Xenopus laevis egg extracts.</a> J Vis Exp. , (2008).</li><li>Willis, J., DeStephanis, D., Patel, Y., Gowda, V., Yan, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Study+of+the+DNA+damage+checkpoint+using+Xenopus+egg+extracts.">Study of the DNA damage checkpoint using Xenopus egg extracts.</a> J Vis Exp. , (2012).</li><li>Sive, H. L., Grainger, R. M., Harland, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Early Development of Xenopus Laevis : A Laboratory Manual. , (2000).</li><li>Rubinfeld, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Binding+of+GSK3&#946;+to+the+APC-&#946;-Catenin+Complex+and+Regulation+of+Complex+Assembly.">Binding of GSK3&#946; to the APC-&#946;-Catenin Complex and Regulation of Complex Assembly.</a> Science. 272, 1023-1026 (1996).</li><li>Salic, A., King, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identifying+small+molecule+inhibitors+of+the+ubiquitin-proteasome+pathway+in+Xenopus+egg+extracts.">Identifying small molecule inhibitors of the ubiquitin-proteasome pathway in Xenopus egg extracts.</a> Methods in Enzymology. 399, 567-585 (2005).</li><li>Trinkle-Mulcahy, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identifying+specific+protein+interaction+partners+using+quantitative+mass+spectrometry+and+bead+proteomes.">Identifying specific protein interaction partners using quantitative mass spectrometry and bead proteomes.</a> J Cell Biol. 183, 223-239 (2008).</li><li>Tan, C. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wnt+signalling+pathway+parameters+for+mammalian+cells.">Wnt signalling pathway parameters for mammalian cells.</a> PLoS One. 7, (2012).</li></ol>

The authors declare that they have no competing financial interests.

Xenopus egg extract is a robust biochemical system for investigating &#946;-catenin turnover. The concentration of &#946;-catenin in Xenopus egg extract is ~25 nM&#160;2. Under optimal conditions, the egg extract is capable of degrading &#946;-catenin at a rate of 50-100 nM/hr and is half-maximal at 200 nM24. There are several critical steps for successful reconstitution of &#946;-catenin degradation using Xenopus egg extract. These include 1) generating high quality X...

A correction was made to <a href="http://www.jove.com/video/51425">Reconstitution Of &#946;-catenin Degradation In Xenopus Egg Extract</a>. At the time of publication there were some instances where an incorrect volume notation was used. These instances were corrected from:
2.1.2. Add extract to 1/10 the volume of pelleted antibody or affinity beads (e.g., 20 ml pelleted beads to 200 ml extract). In order to minimize dilution of the extract, withdraw as much liquid from the beads as possible before addition of the extract using gel loading tips with long, tapered tips.
2.2.5. Aliquot the appropriate volumes for degradation assay into pre-chilled microfuge tubes on ice. For radiolabeled &#946;-catenin degradation assays, withdraw 2-5 ml extract for each time point.
3.2.3. At the designated time point, remove 1-5 ml of the sample and mix immediately with SDS sample buffer (5x volume) to stop the reaction. To make sure the degradation reaction is completely terminated, flick tube several times and vortex vigorously.
3.2.4. Perform SDS-PAGE/autoradiography. Run 1 ml equivalents (~50 mg of protein) of the extract for each time point/lane. Degradation of &#946;-catenin in Xenopus egg extract should be evidenced by the time-dependent decrease in intensity of the radiolabeled &#946;-catenin band Figure 2. Quantify results using ImageJ, ImageQuant, or other preferred imaging software if necessary.
4.2.2. Add in vitro-translated &#946;-catenin-luciferase fusion (from 4.1) into prepared Xenopus reaction mix (from 2.2) on ice and mix well as in 3.2.1. NOTE: The activity of the &#946;-catenin luciferase that is added to the extract is typically between 20 - 50,000 relative luminescence units (RLU)/ml of extract (based on measurements obtained from 4.1.2). Starting signal should be approximately 100,000 RLU (2-5 ml of the in vitro-translated &#946;-catenin-luciferase fusion).
to:
2.1.2. Add extract to 1/10 the volume of pelleted antibody or affinity beads (e.g., 20 &#181;l pelleted beads to 200 &#181;l extract). In order to minimize dilution of the extract, withdraw as much liquid from the beads as possible before addition of the extract using gel loading tips with long, tapered tips.
2.2.5. Aliquot the appropriate volumes for degradation assay into pre-chilled microfuge tubes on ice. For radiolabeled &#946;-catenin degradation assays, withdraw 2-5 &#181;l extract for each time point.
3.2.3. At the designated time point, remove 1-5 &#181;l of the sample and mix immediately with SDS sample buffer (5x volume) to stop the reaction. To make sure the degradation reaction is completely terminated, flick tube several times and vortex vigorously.
3.2.4. Perform SDS-PAGE/autoradiography. Run 1 &#181;l equivalents (~50 mg of protein) of the extract for each time point/lane. Degradation of &#946;-catenin in Xenopus egg extract should be evidenced by the time-dependent decrease in intensity of the radiolabeled &#946;-catenin band Figure 2. Quantify results using ImageJ, ImageQuant, or other preferred imaging software if necessary.
4.2.2. Add in vitro-translated &#946;-catenin-luciferase fusion (from 4.1) into prepared Xenopus reaction mix (from 2.2) on ice and mix well as in 3.2.1. NOTE: The activity of the &#946;-catenin luciferase that is added to the extract is typically between 20 - 50,000 relative luminescence units (RLU)/&#181;l of extract (based on measurements obtained from 4.1.2). Starting signal should be approximately 100,000 RLU (2-5 &#181;l of the in vitro-translated &#946;-catenin-luciferase fusion).

Erratum: Reconstitution Of &#946;-catenin Degradation In Xenopus Egg Extract

Xenopus laevis egg extract has been used extensively to study many cell biological processes including cytoskeletal dynamics, nuclear assembly and import, apoptosis, ubiquitin metabolism, cell cycle progression, signal transduction, and protein turnover1-17. The Xenopus egg extract system is amenable to the biochemical analysis of a legion of cellular processes because egg extract represents essentially undiluted cytoplasm that contains all the essential cytoplasmic components necessary to execute these processes and enable investigation. Large quantities of egg extract can be prepared at one time for biochemical manipulations that require large amounts of material (e.g., protein purification or high-throughput screening)18-20. Another advantage is that the concentration of specific proteins in Xenopus egg extract can be precisely adjusted by addition of recombinant protein and/or immunodepletion of endogenous proteins in contrast to transfection of plasmid DNA where expression of the protein of interest is difficult to control. In addition, the lack of available recombinant proteins can be overcome by the addition of transcripts encoding the protein of interest, taking advantage of the freshly prepared Xenopus egg extract&#8217;s high capacity to translate exogenously added mRNA.
The regulation of protein degradation is critical for the control of many cellular pathways and processes21. Xenopus egg extract has been used extensively to study protein degradation as the system allows for multiple ways to monitor protein turnover without confounding influences of transcription and translation. The Wnt signaling pathway is a highly conserved signaling pathway that plays critical roles in development and disease. The turnover of &#946;-catenin, the major effector of the Wnt pathway, is highly regulated, and an increased steady-state level of &#946;-catenin is critical for the activation of Wnt target genes. The importance of &#946;-catenin degradation is highlighted by the fact that mutations in the Wnt pathway that inhibit &#946;-catenin degradation found in ~90% of all sporadic cases of colorectal cancer22. &#946;-catenin degradation by components of the Wnt pathway can be faithfully recapitulated in Xenopus egg extract to study the mechanism of its turnover as well as to identify novel small molecule modulators of its degradation2,19,20,23-29 .
Methods for the preparation of Xenopus egg extract for studying the cell cycle have been described in previous JoVE publications30-32. The current protocol describes a modification of these methods and is optimized for the degradation of [35S]-radiolabeled &#946;-catenin and luciferase-tagged &#946;-catenin in Xenopus egg extract. The radiolabeled degradation assay allows for direct visualization of protein levels via autoradiography. [35S]methionine is incorporated into the protein of interest using an in vitro translation reaction that can then be directly added to a degradation reaction. In addition, the radiolabeled protein turnover assay does not require an antibody against the protein of interest or an epitope tag, which can influence protein stability. Because even small changes in protein levels, as reflected in changes in the intensity of the radiolabeled protein band, are readily visualized by autoradiography, the [35S]-radiolabeled degradation assay represents a very useful method for visualization of protein turnover2.
Fusion of &#946;-catenin to firefly luciferase (hereafter referred to as simply &#34;luciferase&#34;) allows for precise and facile quantitative measurements of protein levels, in order to determine the kinetic properties of &#946;-catenin turnover19,20 . A major advantage of the luciferase assay is that it provides a strong quantitative system that is easily scaled up. The following protocol provides simple methods for assaying &#946;-catenin degradation and a robust, efficient, and effective method for high-throughput screening of novel &#946;-catenin modulators.

<table border="0" cellpadding="0" cellspacing="0" style="width:709px;" width="710">
	<colgroup>
		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Name of Reagent/ Material</td>
			<td style="width:132px;">Company</td>
			<td style="width:119px;">Catalog Number</td>
			<td style="width:243px;">Comments/Description</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Pregnant Mare Serum Gonadotropin (PMSG)</td>
			<td style="width:132px;">ProSpec</td>
			<td style="width:119px;">hor-272-A</td>
			<td>Reconstituted with distilled water before use.&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Human Chorionic Gonadotropin</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">CG10-10VL</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Potassium Chloride</td>
			<td style="width:132px;">Fisher</td>
			<td style="width:119px;">BP366-1</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Sodium Chloride</td>
			<td style="width:132px;">Research Products International</td>
			<td style="width:119px;">S23020-5000.0</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Magnesium Chloride</td>
			<td style="width:132px;">Fisher</td>
			<td style="width:119px;">BP214-500</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Calcium Chloride</td>
			<td style="width:132px;">Acros Organics</td>
			<td style="width:119px;">AC42352-5000</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">HEPES</td>
			<td style="width:132px;">Fisher</td>
			<td style="width:119px;">BP310-1</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Cysteine</td>
			<td style="width:132px;">Acros Organics</td>
			<td style="width:119px;">AC17360-1000</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Leupeptin</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">L2884-10MG</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Aprotinin</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">A1153-10MG</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Pepstatin</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">P4265-5MG</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Cytochalasin B</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">C8273-10MG</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">3 mL syringe: Luer Lock tip</td>
			<td style="width:132px;">Becton Dickinson</td>
			<td style="width:119px;">309657</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">27G1 needle</td>
			<td style="width:132px;">Becton Dickinson</td>
			<td style="width:119px;">305109</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">96 well solid white polystyrene microplate, round bottomed</td>
			<td style="width:132px;">Corning</td>
			<td style="width:119px;">3605</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Steady-Glo Luciferase assay system</td>
			<td style="width:132px;">Promega</td>
			<td style="width:119px;">E2520</td>
			<td>Store long-term at -80, can store for up to 1 month at -20</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">TNT Sp6 coupled reticulocyte lysate system</td>
			<td style="width:132px;">Promega</td>
			<td style="width:119px;">L4600</td>
			<td></td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">TNT Sp6 High-yield Wheat Germ protein expression system</td>
			<td style="width:132px;">Promega</td>
			<td style="width:119px;">L3260</td>
			<td>Generally higher yield than reticulocyte lysate&#160;</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">EasyTag Express Protein Labeling Mix [S35]</td>
			<td style="width:132px;">Perkin Elmer</td>
			<td style="width:119px;">NEG772007MC</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Creatine phosphate</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">27920-5G</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">ATP</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">A2383-5G</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Creatine phosphokinase</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">C3755-35KU</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Dimethyl Sulfoxide (DMSO)</td>
			<td style="width:132px;">Sigma</td>
			<td style="width:119px;">D8418-50ML</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Dual-Glo luciferase assay system</td>
			<td style="width:132px;">Promega</td>
			<td style="width:119px;">E2920</td>
			<td>Same storage conditions as Steady-Glo</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">50 mL Centrifuge tubes</td>
			<td style="width:132px;">Fisher Scientific</td>
			<td style="width:119px;">0556214D</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sorvall SS-34 fixed angle rotor</td>
			<td style="width:132px;">Thermo Scientific</td>
			<td style="width:119px;">28020</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">115 V 50/60 Hz Minicentrifuge</td>
			<td style="width:132px;">Fisher Scientific</td>
			<td style="width:119px;">05-090-128</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">mMessage mMachine Sp6 kit</td>
			<td style="width:132px;">Ambion</td>
			<td style="width:119px;">AM1340</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Anti-Firefly Luciferase antibody</td>
			<td style="width:132px;">Abcam</td>
			<td style="width:119px;">ab16466</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Anti-GSK3 antibody</td>
			<td style="width:132px;">BD Transduction Laboratories</td>
			<td style="width:119px;">610201</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;"></td>
			<td style="width:132px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;"></td>
			<td style="width:132px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;"></td>
			<td style="width:132px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;"></td>
			<td style="width:132px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Name of Equipment</td>
			<td style="width:132px;">Company</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">FLUOStar Optima</td>
			<td style="width:132px;">BMG Labtech</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Sorvall RC-6 Plus Centrifuge</td>
			<td style="width:132px;">Thermo Scientific</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">16&#176;C Incubator</td>
			<td style="width:132px;">Percival Scientific</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
	</tbody>
</table>

reconstitution of &#946;-catenin degradation in xenopus egg extract

Xenopus laevis egg extract is a well-characterized, robust system for studying the biochemistry of diverse cellular processes. Xenopus egg extract has been used to study protein turnover in many cellular contexts, including the cell cycle and signal transduction pathways1-3. Herein, a method is described for isolating Xenopus egg extract that has been optimized to promote the degradation of the critical Wnt pathway component, &beta;-catenin. Two different methods are described to assess &beta;-catenin protein degradation in Xenopus egg extract. One method is visually informative ([35S]-radiolabeled proteins), while the other is more readily scaled for high-throughput assays (firefly luciferase-tagged fusion proteins). The techniques described can be used to, but are not limited to, assess &beta;-catenin protein turnover and identify molecular components contributing to its turnover. Additionally, the ability to purify large volumes of homogenous Xenopus egg extract combined with the quantitative and facile readout of luciferase-tagged proteins allows this system to be easily adapted for high-throughput screening for modulators of &beta;-catenin degradation.

A schematic of &#946;-catenin degradation in Xenopus egg extract is shown in Figure 2A. 35S-labeled &#946;-catenin was incubated in Xenopus egg extract, aliquots (1 ml extract equivalent) were removed at the appropriate times, and samples were subjected to SDS-PAGE followed by autoradiography. &#946;-catenin degradation by components of the Wnt pathway is mediated by the ubiquitin-proteasome system2, and degradation of [35S]-radiolabeled &#946;-catenin i...

Watch this Scientific Journal Video about Reconstitution Of Î²-catenin Degradation In Xenopus Egg Extract at JoVE.com

Reconstitution Of &amp;#946;-catenin Degradation In Xenopus Egg Extract

A method is described for analyzing protein degradation using radiolabeled and luciferase-fusion proteins in Xenopus egg extract and its adaptation for high-throughput screening for small molecule modulators of protein degradation.

Reconstitution Of &#946;-catenin Degradation In Xenopus Egg Extract

Xenopus laevis egg extract is a well-characterized, robust system for studying the biochemistry of diverse cellular processes. Xenopus egg extract has ...

reconstitution-of-catenin-degradation-in-xenopus-egg-extract

Research

Education

JoVE Journal

JoVE Core

Molecular Biology

Biology

Unit 1

Translation: RNA to Protein

SCIENTISTS IN ACTION

12.0K Views.  Vanderbilt University Medical Center. A method is described for analyzing protein degradation using radiolabeled and luciferase-fusion proteins in Xenopus egg extract and its adaptation for high-throughput screening for small molecule modulators of protein degradation.

Video: Reconstitution Of &#946;-catenin Degradation In Xenopus Egg Extract

Preparation and Fractionation of Xenopus laevis Egg Extracts

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.

Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical ...

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm ...

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

The protocol presented here is designed to study the activation of the large conductance, voltage- and Ca2+-activated K+ (BK) channels. The protocol may also be used to study the structure-function relationship for other ion channels and neurotransmitter receptors1. BK channels are widely expressed in different tissues and have been implicated in many physiological functions, including regulation of smooth muscle contraction, frequency tuning of inner hair cells and regulation of neurotransmitter release2-6. BK channels are activated by membrane depolarization and by intracellular Ca2+ and Mg2+ 6-9. Therefore, the protocol is designed to control both the membrane voltage and the intracellular solution. In this protocol, messenger RNA of BK channels is injected into Xenopus laevis oocytes (stage V-VI) followed by 2-5 days of incubation at 18&deg;C10-13. Membrane patches that contain single or multiple BK channels are excised with the inside-out configuration using patch clamp techniques10-13. The intracellular side of the patch is perfused with desired solutions during recording so that the channel activation under different conditions can be examined. To summarize, the mRNA of BK channels is injected into Xenopus laevis oocytes to express channel proteins on the oocyte membrane; patch clamp techniques are used to record currents flowing through the channels under controlled voltage and intracellular solutions.

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

Ionic current of BK channels is recorded using patch clamp techniques. BK channels are expressed in Xenopus oocytes by injecting messenger RNA. The intracellular solution during patch clamp recordings is controlled by a perfusion system.

The protocol presented here is designed to study the activation of the large conductance, voltage- and Ca2+-activated K+ (BK) channels. The protocol may ...

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

On a daily basis, cells are subjected to a variety of endogenous and environmental insults. To combat these insults, cells have evolved DNA damage checkpoint signaling as a surveillance mechanism to sense DNA damage and direct cellular responses to DNA damage. There are several groups of proteins called sensors, transducers and effectors involved in DNA damage checkpoint signaling (Figure 1). In this complex signaling pathway, ATR (ATM and Rad3-related) is one of the major kinases that can respond to DNA damage and replication stress. Activated ATR can phosphorylate its downstream substrates such as Chk1 (Checkpoint kinase 1). Consequently, phosphorylated and activated Chk1 leads to many downstream effects in the DNA damage checkpoint including cell cycle arrest, transcription activation, DNA damage repair, and apoptosis or senescence (Figure 1). When DNA is damaged, failing to activate the DNA damage checkpoint results in unrepaired damage and, subsequently, genomic instability. The study of the DNA damage checkpoint will elucidate how cells maintain genomic integrity and provide a better understanding of how human diseases, such as cancer, develop.
Xenopus laevis egg extracts are emerging as a powerful cell-free extract model system in DNA damage checkpoint research. Low-speed extract (LSE) was initially described by the Masui group1. The addition of demembranated sperm chromatin to LSE results in nuclei formation where DNA is replicated in a semiconservative fashion once per cell cycle.
The ATR/Chk1-mediated checkpoint signaling pathway is triggered by DNA damage or replication stress 2. Two methods are currently used to induce the DNA damage checkpoint: DNA damaging approaches and DNA damage-mimicking structures 3. DNA damage can be induced by ultraviolet (UV) irradiation, &gamma;-irradiation, methyl methanesulfonate (MMS), mitomycin C (MMC), 4-nitroquinoline-1-oxide (4-NQO), or aphidicolin3, 4. MMS is an alkylating agent that inhibits DNA replication and activates the ATR/Chk1-mediated DNA damage checkpoint 4-7. UV irradiation also triggers the ATR/Chk1-dependent DNA damage checkpoint 8. The DNA damage-mimicking structure AT70 is an annealed complex of two oligonucleotides poly-(dA)70 and poly-(dT)70. The AT70 system was developed in Bill Dunphy's laboratory and is widely used to induce ATR/Chk1 checkpoint signaling 9-12.
Here, we describe protocols (1) to prepare cell-free egg extracts (LSE), (2) to treat Xenopus sperm chromatin with two different DNA damaging approaches (MMS and UV), (3) to prepare the DNA damage-mimicking structure AT70, and (4) to trigger the ATR/Chk1-mediated DNA damage checkpoint in LSE with damaged sperm chromatin or a DNA damage-mimicking structure.

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Xenopus egg extract is a useful model system to investigate the DNA damage checkpoint. This protocol is for the preparation of Xenopus egg extracts and DNA damage checkpoint inducing reagents. These techniques are adaptable to a variety of DNA damaging approaches in the study of the DNA damage checkpoint signaling.

On a daily basis, cells are subjected to a variety of endogenous and environmental insults. To combat these insults, cells have evolved DNA damage ...

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Many organisms localize mRNAs to specific subcellular destinations to spatially and temporally control gene expression. Recent studies have demonstrated that the majority of the transcriptome is localized to a nonrandom position in cells and embryos. One approach to identify localized mRNAs is to biochemically purify a cellular structure of interest and to identify all associated transcripts. Using recently developed high-throughput sequencing technologies it is now straightforward to identify all RNAs associated with a subcellular structure. To facilitate transcript identification it is necessary to work with an organism with a fully sequenced genome. One attractive system for the biochemical purification of subcellular structures are egg extracts produced from the frog Xenopus laevis. However, X. laevis currently does not have a fully sequenced genome, which hampers transcript identification. In this article we describe a method to produce egg extracts from a related frog, X. tropicalis, that has a fully sequenced genome. We provide details for microtubule polymerization, purification and transcript isolation. While this article describes a specific method for identification of microtubule-associated transcripts, we believe that it will be easily applied to other subcellular structures and will provide a powerful method for identification of localized RNAs.

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

We describe the collection of unfertilized Xenopus tropicalis eggs and production of a meiosis II-arrested egg extract. This egg extract can be used to purify microtubules and microtubule-associated RNAs.

Many  organisms localize mRNAs to specific subcellular destinations to spatially and  temporally control gene expression. Recent studies have demonstrated ...

Facial Transplants in Xenopus laevis Embryos

Craniofacial birth defects occur in 1 out of every 700 live births, but etiology is rarely known due to limited understanding of craniofacial development. To identify where signaling pathways and tissues act during patterning of the developing face, a 'face transplant' technique has been developed in embryos of the frog Xenopus laevis. A region of presumptive facial tissue (the "Extreme Anterior Domain" (EAD)) is removed from a donor embryo at tailbud stage, and transplanted to a host embryo of the same stage, from which the equivalent region has been removed. This can be used to generate a chimeric face where the host or donor tissue has a loss or gain of function in a gene, and/or includes a lineage label. After healing, the outcome of development is monitored, and indicates roles of the signaling pathway within the donor or surrounding host tissues. Xenopus is a valuable model for face development, as the facial region is large and readily accessible for micromanipulation. Many embryos can be assayed, over a short time period since development occurs rapidly. Findings in the frog are relevant to human development, since craniofacial processes appear conserved between Xenopus and mammals.

Facial Transplants in Xenopus laevis Embryos

A technique for transplanting "Extreme Anterior Domain" facial tissue between Xenopus laevis embryos has been developed. Tissue can be moved from one gene expression background into another, allowing the study of local requirements for craniofacial development and for signaling interactions between facial regions.

Craniofacial birth defects occur in 1 out of every 700 live births, but etiology is rarely known due to limited understanding of craniofacial development. ...

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

The cut-open oocyte Vaseline gap (COVG) voltage clamp technique allows for analysis of electrophysiological and kinetic properties of heterologous ion channels in oocytes. Recordings from the cut-open setup are particularly useful for resolving low magnitude gating currents, rapid ionic current activation, and deactivation. The main benefits over the two-electrode voltage clamp (TEVC) technique include increased clamp speed, improved signal-to-noise ratio, and the ability to modulate the intracellular and extracellular milieu.
Here, we employ the human cardiac sodium channel (hNaV1.5), expressed in Xenopus oocytes, to demonstrate the cut-open setup and protocol as well as modifications that are required to add voltage clamp fluorometry capability.
The properties of fast activating ion channels, such as hNaV1.5, cannot be fully resolved near room temperature using TEVC, in which the entirety of the oocyte membrane is clamped, making voltage control difficult. However, in the cut-open technique, isolation of only a small portion of the cell membrane allows for the rapid clamping required to accurately record fast kinetics while preventing channel run-down associated with patch clamp techniques.
In conjunction with the COVG technique, ion channel kinetics and electrophysiological properties can be further assayed by using voltage clamp fluorometry, where protein motion is tracked via cysteine conjugation of extracellularly applied fluorophores, insertion of genetically encoded fluorescent proteins, or the incorporation of unnatural amino acids into the region of interest1. This additional data yields kinetic information about voltage-dependent conformational rearrangements of the protein via changes in the microenvironment surrounding the fluorescent molecule.

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

The cut-open Vaseline gap approach is used to obtain low noise recordings of ionic and gating currents from voltage-dependent ion channels expressed in Xenopus oocytes with high resolution of fast channel kinetics. With minor modification, voltage clamp fluorometry can be coupled to the cut-open oocyte protocol.

The cut-open oocyte Vaseline gap (COVG) voltage clamp technique allows for analysis of electrophysiological and kinetic properties of heterologous ion ...

Quantification of Orofacial Phenotypes in Xenopus

Xenopus has become an important tool for dissecting the mechanisms governing craniofacial development and defects. A method to quantify orofacial development will allow for more rigorous analysis of orofacial phenotypes upon abrogation with substances that can genetically or molecularly manipulate gene expression or protein function. Using two dimensional images of the embryonic heads, traditional size dimensions-such as orofacial width, height and area- are measured. In addition, a roundness measure of the embryonic mouth opening is used to describe the shape of the mouth. Geometric morphometrics of these two dimensional images is also performed to provide a more sophisticated view of changes in the shape of the orofacial region. Landmarks are assigned to specific points in the orofacial region and coordinates are created. A principle component analysis is used to reduce landmark coordinates to principle components that then discriminate the treatment groups. These results are displayed as a scatter plot in which individuals with similar orofacial shapes cluster together. It is also useful to perform a discriminant function analysis, which statistically compares the positions of the landmarks between two treatment groups. This analysis is displayed on a transformation grid where changes in landmark position are viewed as vectors. A grid is superimposed on these vectors so that a warping pattern is displayed to show where significant landmark positions have changed. Shape changes in the discriminant function analysis are based on a statistical measure, and therefore can be evaluated by a p-value. This analysis is simple and accessible, requiring only a stereoscope and freeware software, and thus will be a valuable research and teaching resource.

Quantification of Orofacial Phenotypes in Xenopus

A method to quantify the orofacial size and shape of Xenopus laevis embryos has been developed. In this protocol, traditional size measurements are combined with geometric morphometrics to allow for more sophisticated analyses of orofacial development and defects.

Xenopus has become an important tool for dissecting the mechanisms governing craniofacial development and defects. A method to quantify orofacial ...

Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein

The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a unique channel-forming member of the ATP Binding Cassette (ABC) superfamily of transporters. The phosphorylation and nucleotide dependent chloride channel activity of CFTR has been frequently studied in whole cell systems and as single channels in excised membrane patches. Many Cystic Fibrosis-causing mutations have been shown to alter this activity. While a small number of purification protocols have been published, a fast reconstitution method that retains channel activity and a suitable method for studying population channel activity in a purified system have been lacking. Here rapid methods are described for purification and functional reconstitution of the full-length CFTR protein into proteoliposomes of defined lipid composition that retains activity as a regulated halide channel. This reconstitution method together with a novel flux-based assay of channel activity is a suitable system for studying the population channel properties of wild type CFTR and the disease-causing mutants F508del- and G551D-CFTR. Specifically, the method has utility in studying the direct effects of phosphorylation, nucleotides and small molecules such as potentiators and inhibitors on CFTR channel activity. The methods are also amenable to the study of other membrane channels/transporters for anionic substrates.

Described here is a rapid and effective procedure for functional reconstitution of purified wild-type and mutant CFTR protein that preserves activity for this chloride channel, which is defective in Cystic Fibrosis. Iodide efflux from reconstituted proteoliposomes mediated by CFTR allows studies of channel activity and the effects of small molecules.

The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is a unique channel-forming member of the ATP Binding Cassette (ABC) superfamily of ...

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Regulation of protein abundance is crucial to virtually every cellular process. Protein abundance reflects the integration of the rates of protein synthesis and protein degradation. Many assays reporting on protein abundance (e.g., single-time point western blotting, flow cytometry, fluorescence microscopy, or growth-based reporter assays) do not allow discrimination of the relative effects of translation and proteolysis on protein levels. This article describes the use of cycloheximide chase followed by western blotting to specifically analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast). In this procedure, yeast cells are incubated in the presence of the translational inhibitor cycloheximide. Aliquots of cells are collected immediately after and at specific time points following addition of cycloheximide. Cells are lysed, and the lysates are separated by polyacrylamide gel electrophoresis for western blot analysis of protein abundance at each time point. The cycloheximide chase procedure permits visualization of the degradation kinetics of the steady state population of a variety of cellular proteins. The procedure may be used to investigate the genetic requirements for and environmental influences on protein degradation.

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).