Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk Metabolomics

420 Views

•

03:39 min

•

November 8th, 2024

DOI :

10.3791/66849-v

November 8th, 2024

•

Darian T. Carroll¹, Sarah R. Lindsley², Melissa Kirigiti², Alex Buko³, Angela Jones⁴, Paul Kievit², Maureen Gannon¹^,⁵^,⁶^,⁷

¹Department of Molecular Physiology and Biophysics, Vanderbilt University, ²Division of Metabolic Health and Disease, Oregon National Primate Research Center, ³Human Metabolome Technologies (HMT) America, ⁴Vanderbilt Technologies for Advanced Genomics, Vanderbilt University Medical Center, ⁵Department of Medicine, Vanderbilt University Medical Center, ⁶Department of Veterans Affairs Tennessee Valley, ⁷Department of Cell and Developmental Biology, Vanderbilt University

Summary

Explore More Videos

Frozen Primate Fetal Islets

Single Nuclei RNA sequencing

Embryonic Development

Postnatal Function

Metabolome Analysis

Genome Interaction

Multi omic Experiments

Batch Effects

Transcriptomic Analysis

Epigenomic Landscapes

Capillary Electrophoresis mass Spectrometry

Chapters in this video

0:00

Inroduction

1:09

Cellular Fractionation of Rhesus Macaque Pancreatic Islets for Single‐Cell RNA Sequencing and Metabolomics

Related Videos

08:14

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

7.5K Views

07:04

Effective Isolation of Functional Islets from Neonatal Mouse Pancreas

10.0K Views

07:06

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

46.1K Views

07:21

Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

11.0K Views

06:43

Intravenous and Intra-amniotic In Utero Transplantation in the Murine Model

9.4K Views

06:43

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo

10.1K Views

06:58

Culturing and Measuring Fetal and Newborn Murine Long Bones

8.1K Views

07:12

Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption

5.4K Views

07:44

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

1.2K Views

11:44

Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification

3.1K Views